Abstract: Objective: To explore the clinical value of treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA) and rapid plasma reactin ring card test (RPR) in diagnosis of Treponema pallidum infection. Methods: Treponema pallidum infection was detected by treponema pallidum particle assay (TPPA), TP-ELISA and RPR in 193 suspected Treponema pallidum infection patients, and the diagnostic value of TP-ELISA and RPR was evaluated by taking the results of TPPA as gold standard. Results: In 193 suspected Treponema pallidum infection patients, TPPA confirmed 156 cases. The S/CO value of TP-ELISA test and antibody titer of RPR test in Treponema pallidum infection positive patients were all obivously higher than Treponema pallidum infection negative patients (P<0.05). The sensitivity and negative predictive value of TP-ELISA in diagnosis of Treponema pallidum infection were significantly higher than RPR, while the omission diagnostic rate was significantly lower (P<0.05). There was no statistical significance in specificity, misdiagnosis rate and positive predictive value between TP-ELISA and RPR in diagnosis of Treponema pallidum infection (P>0.05). The consistency between TP-ELISA and TPPA in diagnosis of Treponema pallidum infection was 0.950, and that between RPR and TPPA was 0.531. Conclusions: Compared with RPR test, the sensitivity of TP-ELISA in diagnosis of Treponema pallidum infection is high and can reduce missed diagnosis, so it is worthy of clincial application.

Key words: Treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA), Rapid plasma reactin ring card test (RPR), Treponema pallidum particle assay(TPPA), Treponema pallidum, Diagnosis

摘要： 目的:探讨梅毒螺旋体酶联免疫吸附试验（TP-ELISA）、快速血浆反应素环状卡片试验（RPR）诊断梅毒螺旋体感染的临床价值。方法:193例疑似梅毒螺旋体感染患者分别采用梅毒螺旋体明胶凝集法（TPPA）、TP-ELISA法、RPR法检测梅毒螺旋体感染情况,并以TPPA诊断结果为金标准评价TP-ELISA法和RPR法诊断梅毒螺旋体感染的临床价值。结果:193例疑似梅毒感染患者TPPA试验确诊156例,梅毒螺旋体感染阳性患者TP-ELISA法检测的S/CO值、RPR法检测的抗体滴度均显著高于梅毒螺旋体阴性患者,差异具有统计学意义（P<0.05）。TP-ELISA法诊断梅毒螺旋体感染的灵敏度和阴性预测值明显高于RPR法（P<0.05）,漏诊率明显低于RPR法（P<0.05）;TP-ELISA法和RPR法诊断梅毒螺旋体感染的特异度、误诊率、阳性预测值比较,差异无统计学意义（P>0.05）。TP-ELISA法诊断梅毒螺旋体感染与TPPA诊断结果的一致性Kappa值为0.950,RPR法与TPPA诊断结果的一致性Kappa值为0.531。结论:与RPR法比较,TP-ELISA法诊断梅毒螺旋体感染的敏感性较高,且能减少漏诊状况,值得临床推广应用。

关键词: 梅毒螺旋体联免疫吸附试验（TP-ELISA）, 快速血浆反应素环状卡片试验（RPR）, 梅毒螺旋体明胶凝集法（TPPA）, 梅毒螺旋体, 诊断