Correlation between EID2B expression and cell migration in colon cancer

Abstract

Abstract: Objective To investigate whether EID2B affects tumor cell migration within the colon adenocarcinoma (COAD) immune microenvironment, and to provide a reference for subsequent research in this field. Methods The expression levels of EID2B in COAD were analyzed using the TCGA database, along with its relationship to clinical staging and survival curves; the HPA database was interrogated for analyze the protein expression levels of EID2B; the TIMER database was utilized to analyze the correlation between EID2B and immune cell infiltration; the TCGA database was applied to enrichment analysis of EID2B biological processes; in vitro experiments involved the design of siRNA transfection in THP-1 induced macrophages to knock down EID2B expression, with RT-qPCR detecting the efficiency of EID2B knockdown. Scratch assays and Transwell assays were conducted to evaluate the impact on the migration ability of HCT116 cells. Results EID2B is underexpressed in COAD (P<0.05), correlated with lymph node metastasis (P<0.05), and significantly correlated with macrophage infiltration (P<0.05). The culture supernatant from EID2B knockdown macrophages promoted the migration of HCT116 cells (P<0.05). Conclusion EID2B underexpression is associated with lymph node metastasis in COAD, and knocking down EID2B promotes tumor migration.

Key words: EID2B, colon adenocarcinoma, prognosis, immune cell infiltration, macrophage

摘要： 目的探究EID2B在结肠癌（COAD）免疫微环境中对肿瘤细胞迁移的影响。方法通过TCGA数据库分析EID2B在COAD中的表达水平,及其与临床分期及生存曲线的关系;HPA数据库分析EID2B的蛋白表达水平;TIMER数据库分析EID2B与免疫细胞浸润的相关性;TCGA数据库进行EID2B生物学过程富集分析;利用体外实验设计siRNA转染THP-1诱导的巨噬细胞以敲减EID2B表达,RT-qPCR检测EID2B敲减效率。划痕实验、Transwell实验评估对HCT116细胞迁移能力的影响。结果 EID2B在COAD中呈低表达（P<0.05）,与淋巴结转移和M2巨噬细胞的浸润水平呈负相关（P<0.05）;敲减EID2B的巨噬细胞培养上清可促进HCT116的迁移（P<0.05）。结论 EID2B低表达与COAD淋巴结转移相关,敲减EID2B可促进肿瘤迁移。

关键词: EID2B, 结肠癌, 预后, 免疫细胞浸润, 巨噬细胞

CLC Number:

R735.35

ZHAO Wei, WU Yudan, WANG Yixiao, LIU Qiuyan, HOU Zhiping. Correlation between EID2B expression and cell migration in colon cancer[J]. Journal of Chengde Medical University, 2025, 42(6): 462-467.

赵巍, 吴豫丹, 王意霄, 刘秋艳, 侯志平. EID2B在结肠癌中的表达及与细胞迁移的相关性分析[J]. 承德医学院学报, 2025, 42(6): 462-467.

Add to citation manager EndNote|Ris|BibTeX

URL: http://tougao.cdmc.edu.cn:8088/EN/

http://tougao.cdmc.edu.cn:8088/EN/Y2025/V42/I6/462

References

[1] Wang Y, Yan Q, Fan C, et al.Overview and countermeasures of cancer burden in China[J]. Sci China Life Sci, 2023, 66(11): 2515-2526.
[2] Zhou Y, Song K, Chen Y, et al.Burden of six major types of digestive system cancers globally and in China[J]. Chin Med J (Engl), 2024, 137(16): 1957-1964.
[3] de Visser KE, Joyce JA. The evolving tumor microenvironment: From cancer initiation to metastatic outgrowth[J]. Cancer Cell, 2023, 41(3): 374-403.
[4] Lv B, Wang Y, Ma D, et al.Immunotherapy: Reshape the Tumor Immune Microenvironment[J]. Front Immunol, 2022, 13: 844142.
[5] Liu Y, Zhang Q, Xing B, et al.Immune phenotypic linkage between colorectal cancer and liver metastasis[J]. Cancer Cell, 2022, 40(4): 424-437.
[6] Davern M, Donlon NE, Power R, et al.The tumour immune microenvironment in oesophageal cancer[J]. Br J Cancer, 2021, 125(4): 479-494.
[7] Lei X, Lei Y, Li JK, et al.Immune cells within the tumor microenvironment: Biological functions and roles in cancer immunotherapy[J]. Cancer Lett, 2020, 470: 126-133.
[8] Xiang Y, Zhu L, He Z, et al.EID3 Promotes Glioma Cell Proliferation and Survival by Inactivating AMPKα1[J]. J Korean Neurosurg Soc, 2022, 65(6): 790-800.
[9] Wang Y, Luo S, Wang Y, et al.EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway[J]. Oxid Med Cell Longev, 2022, 2022: 5941562.
[10] Zhu Y, Wang Z, Li Y, et al.The Role of CREBBP/EP300 and Its Therapeutic Implications in Hematological Malignancies[J]. Cancers (Basel), 2023, 15(4): 1219.
[11] Hudson JJ, Bednarova K, Kozakova L, et al.Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families[J]. PLoS One, 2011, 6(2): e17270.
[12] Sasajima Y, Tanaka H, Miyake S, et al.A novel EID family member, EID-3, inhibits differentiation and forms a homodimer or heterodimer with EID-2[J]. Biochem Biophys Res Commun, 2005, 333(3): 969-975.
[13] Li T, Fu J, Zeng Z, et al.TIMER2.0 for analysis of tumor-infiltrating immune cells[J]. Nucleic Acids Res, 2020, 48(W1): W509-W514.
[14] Li Y, Yang T, Lai T, et al.CDCP: a visualization and analyzing platform for single-cell datasets[J]. J Genet Genomics, 2022, 49(7): 689-692.
[15] Yang S, Kim CY, Hwang S, et al.COEXPEDIA: exploring biomedical hypotheses via co-expressions associated with medical subject headings (MeSH)[J]. Nucleic Acids Res, 2017, 45(D1): D389-D396.
[16] Schmitt M, Greten FR.The inflammatory pathogenesis of colorectal cancer[J]. Nat Rev Immunol, 2021, 21(10): 653-667.
[17] Al Zein M, Boukhdoud M, Shammaa H, et al.Immunotherapy and immunoevasion of colorectal cancer[J]. Drug Discov Today, 2023, 28(9): 103669.
[18] Zhang W, Wang M, Ji C, et al.Macrophage polarization in the tumor microenvironment: Emerging roles and therapeutic potentials[J]. Biomed Pharmacother, 2024, 177: 116930.
[19] Fu LQ, Du WL, Cai MH, et al.The roles of tumor-associated macrophages in tumor angiogenesis and metastasis[J]. Cell Immunol, 2020, 353: 104119.
[20] Ge W, Wu W.Influencing Factors and Significance of Tumor-associated Macrophage Polarization in Tumor Microenvironment[J]. Zhongguo Fei Ai Za Zhi, 2023, 26(3): 228-237.
[21] Wang H, Tian T, Zhang J. Tumor-Associated Macrophages (TAMs) in Colorectal Cancer (CRC): From Mechanism to Therapy and Prognosis[J]. Int J Mol Sci, 2021, 22(16): 8470.
[22] Dong C, Hui P, Wu Z, et al.CircRNA LOC729852 promotes bladder cancer progression by regulating macrophage polarization and recruitment via the miR-769-5p/IL-10 axis[J]. J Cell Mol Med, 2024, 28(7): e18225.
[23] Zhou L, Li J, Liao M, et al.LncRNA MIR155HG induces M2 macrophage polarization and drug resistance of colorectal cancer cells by regulating ANXA2[J]. Cancer Immunol Immunother, 2022, 71(5): 1075-1091.