Abstract: Objective To explore the expression relationship and significance of intramedullary IL-4 and CD34 in rabbit VX2 bone tumors. Methods Thirty Japanese white rabbits were divided into experimental group and control group with 15 rabbits each by random number method. In the experimental group, the left tibia was implanted with VX2 tumor tissue through the tibial plateau to make a bone tumor model. The control group adopted the same operation method but did not implant the VX2 tumor tissue. Closed biopsy was used to extract the bone marrow of the left proximal tibia 7 days and 14 days after operation, and the levels of IL-4 and CD34 in all specimens were detected by enzyme-linked immunosorbent assay (Elisa). At the same time, 14 days after operation, tibia tissue was taken for histopathological examination after execution. Results 30 rabbits were successfully modeled and included in the study. On the 7th day after operation, the intramedullary IL-4 and CD34 contents of rabbits were significantly higher than those before operation, and the difference was statistically significant (P<0.05). On the 14th day after the operation, the IL-4 and CD34 levels were significantly higher than those on the 7th day after the operation. The difference was statistically significant (P<0.05). The pathology showed that the tumor tissue was in the tibial marrow cavity in the bone tumor model group 14 days after the tumor was implanted. Significant tumour growth with tumour cells visible in the bone cortex. Conclusion Within 14 days of modeling rabbit VX2 bone tumor animal model, as the tumor proliferates rapidly, the body's anti-tumor immune function is gradually inhibited, which is manifested by the increased expression of IL-4 and CD34.

Key words: bone tumor, VX2, IL-4, CD34

摘要： 目的 探究兔VX2骨肿瘤微环境中IL-4、CD34的表达关系和意义。方法 将30只日本大耳白兔按随机数法分为实验组与对照组,每组各15只。在实验组左侧胫骨经胫骨平台植入VX2肿瘤组织制成骨肿瘤模型,对照组采取同样的手术方式,但不植入VX2肿瘤组织。术后7d、14d采用封闭活检抽取左侧胫骨近端骨髓,采用酶联免疫吸附实验（Elisa）法检测所有标本中IL-4、CD34含量。同时,在术后14d处死,取胫骨组织行组织病理学检查。结果 实验组、对照组家兔均建模成功,纳入研究。术后7d,家兔髓内IL-4、CD34含量与术前相比均明显升高,差异有统计学意义（P<0.05）。术后14d,IL-4、CD34含量与术后7d相比明显升高,差异有统计学意义（P<0.05）。病理显示：骨肿瘤模型组植瘤14d时,肿瘤组织在胫骨髓腔内显著生长,骨皮质见肿瘤细胞。结论 兔VX2骨肿瘤动物模型建模14d内,随着肿瘤快速增殖,机体抗肿瘤免疫功能逐渐受到抑制,兔骨组织微环境被破坏,表现为IL-4、CD34表达水平升高。

关键词: 骨肿瘤, VX2, IL-4, CD34