Abstract: Objective:To establish the method for simultaneous determination of rhamnosylvitexin, glucosylvitexin, hyperoside and vitexin content in Hawthorn leaf extract by quantitative analysis of multi-components by single marker. Methods:The relative correction factor of rhamnosylvitexin, hyperoside and vitexin were calculated by using glucosylvitexin as internal standard, and the content determination results were compared with the traditional external standard curve method. Results:The relative correction factor between glucosylvitexin and rhamnosylvitexin, hyperoside, vitexin were respectively 0.963, 1.116 and 1.034. There was no obvious difference about the rhamnosylvitexin, hyperoside and vitexin content between quantitative analysis of multi-components by single marker and traditional external standard curve method. Conclusions:The quantitative analysis of multi-components by single marker in this study can be used in simultaneous determination of 4 components in Hawthorn leaf extract.

Key words: Hawthorn leaf extract, Quantitative analysis of multi-components by single marker, Rhamnosylvitexin, Glucosylvitexin, Hyperoside, Vitexin

摘要： 目的: 建立同时测定山楂叶提取物中牡荆素鼠李糖苷、牡荆素葡萄糖苷、金丝桃苷、牡荆素含量的一测多评法。方法: 以牡荆素葡萄糖苷为内标物,计算牡荆素鼠李糖苷、金丝桃苷、牡荆素3个成分的相对校正因子,并将该方法的测量结果与传统外标曲线法的测定结果进行比较。结果: 牡荆素葡萄糖苷与牡荆素鼠李糖苷、金丝桃苷、牡荆素的相对校正因子分别为0.963、1.116、1.034;一测多评法与传统方法测定结果比较,牡荆素鼠李糖苷、金丝桃苷、牡荆素的含量无明显差异。结论: 经方法学验证,本研究建立的一测多评法可用于山楂叶提取物中4种成分含量的同时测定。

关键词: 山楂叶提取物, 一测多评法, 牡荆素鼠李糖苷, 牡荆素葡萄糖苷, 金丝桃苷, 牡荆素