CHANGES OF IRS-1 MRNA EXPRESSION IN PANCREAS OF TYPE 2 DIABETIC RATS AND THE REGULATORY EFFECTS OF SERICIN

Journal of Chengde Medical University ›› 2018, Vol. 35 ›› Issue (4): 279-282.

CHANGES OF IRS-1 MRNA EXPRESSION IN PANCREAS OF TYPE 2 DIABETIC RATS AND THE REGULATORY EFFECTS OF SERICIN

Department of Human Anatomy, Chengde Medical College, Hebei Chengde 067000, China

Received:2018-02-28 Online:2018-08-10 Published:2021-11-21

2型糖尿病大鼠胰腺IRS-1 mRNA表达的变化及丝胶的调控作用^*

李东哲, 王丹丹, 侯丽娜, 宋成军^△, 陈志宏^△

承德医学院人体解剖学教研室,河北承德 067000

通讯作者: △
基金资助:
* 国家自然科学基金资助项目(81441133); 河北省自然科学基金资助项目(H2013406096)

Abstract

Abstract: ObjectiveTo analyze the changes of insulin receptor substrate-1 (IRS-1) mRNA expression in pancreas of type 2 diabetic rats and the regulatory effects of sericin.MethodsMale SD rats were randomly divided into normal control group, diabetic model group and sericin medication group. The type 2 diabetic rats' model was established by high fat and high sugar feed combined streptozotocin injection. The rats in sericin medication group were lavaged with sericin (2.4g/kg) after the diabetic model was successfully established. Glucose oxidase method was used to detect the blood glucose of rats, Real Time Q-PCR to detect the IRS-1 mRNA expression in pancreas.ResultsCompared with rats in normal control group, the blood glucose of rats in diabetic model group increased obviously, the IRS-1 mRNA expression in pancreas decreased obviously (P<0.05). Compared with rats in diabetic model group, the blood glucose of rats in sericin medication group decreased significantly, the IRS-1 mRNA expression in pancreas increased significantly (P<0.05).ConclusionsSericin may reduce blood glucose by up regulating the expression of IRS-1 in pancreas.

Key words: Type 2 diabetes mellitus, Pacnreas, Insulin receptor substrate-1

摘要： 目的: 分析2型糖尿病大鼠胰腺胰岛素受体底物-1（insulin receptor substrate-1,IRS-1）mRNA表达的变化及丝胶的调控作用。方法: 雄性SD大鼠随机分组,正常对照组、糖尿病模型组、丝胶用药组。高脂饲料+高糖饲料喂养联合链脲佐菌素注射建立2型糖尿病大鼠模型,成功建模后,丝胶用药组大鼠给予丝胶（2.4g/kg）灌胃。采用葡萄糖氧化酶法检测各组大鼠的血糖,Real Time Q-PCR法检测各组大鼠胰腺IRS-1 mRNA的表达。结果: 与正常对照组大鼠比较,模型组大鼠的血糖明显升高,胰腺IRS-1 mRNA的表达明显降低（P<0.05）;与模型组大鼠比较,丝胶用药组大鼠的血糖明显降低,胰腺IRS-1 mRNA的表达明显升高（P<0.05）。结论: 丝胶可能通过上调胰腺IRS-1的表达起到降低血糖的作用。

关键词: 2型糖尿病, 胰腺, 胰岛素受体底物-1

CLC Number:

R587.1

LI Dong-zhe, WANG Dan-dan, HOU Li-na, et al. CHANGES OF IRS-1 MRNA EXPRESSION IN PANCREAS OF TYPE 2 DIABETIC RATS AND THE REGULATORY EFFECTS OF SERICIN[J]. Journal of Chengde Medical University, 2018, 35(4): 279-282.

李东哲, 王丹丹, 侯丽娜, 宋成军, 陈志宏. 2型糖尿病大鼠胰腺IRS-1 mRNA表达的变化及丝胶的调控作用^*[J]. 承德医学院学报, 2018, 35(4): 279-282.

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