EXPRESSION AND SIGNIFICANCE OF APOPTOSIS RELATED FACTORS CCAR1 AND PAR-4 IN GASTRIC ADENOCARCINOMA

Abstract

Abstract: Objective: To investigate the expression and significance of cell division cycle and apoptosis regulator protein1 (CCAR1) and prostate apoptosis response-4 gene (Par-4) protein in gastric adenocarcinoma. Methods: SP immunohistochemical staining was used to detect the expression of CCAR1 and Par-4 in 60 gastric adenocarcinoma tissues and 40 paracancerous normal gastric mucosa tissues. The relationships between CCAR1, Par-4 and clinicopathological parameters of gastric adenocarcinoma, as well as the correlation of CCAR1 and Par-4 in gastric adenocarcinoma were analyzed. Results: The positive expression rate of CCAR1 protein in gastric adenocarcinoma tissues was significantly higher, while the Par-4 protein was significantly lower than that of normal gastric mucosa (P<0.05). The CCAR1 protein expression in gastric adenocarcinoma was related to differentiation degree (P<0.05); The expression of par-4 protein was related to differentiation degree, TNM stage and lymph node metastasisa (P<0.05). Spearman correlation analysis showed that no correlation between the expression of CCAR1 protein and Par-4 protein in gastric adenocarcinoma (r=-0.08, P>0.05). Conclusions: Par-4 and CCAR1 involve in the occurrence and progression of gastric adenocarcinoma together, but they may be regulated by different mechanisms.

Key words: Gastric adenocarcinoma, CCAR1, Par-4, Immunohistochemical staining

摘要： 目的: 探讨胃腺癌组织细胞周期与细胞凋亡调控蛋白1（CCAR1）、前列腺凋亡反应基因-4(Par-4)的表达情况及临床意义。方法: 应用免疫组化SP法检测60例胃腺癌组织和40例癌旁正常胃黏膜中CCAR1蛋白、Par-4蛋白的表达情况,分析二者与胃腺癌临床病理参数的关系及胃腺癌组织二者表达的相关性。结果: CCAR1蛋白在胃腺癌组织中的阳性表达率明显高于正常胃黏膜,Par-4蛋白在胃腺癌组织中的阳性表达率明显低于正常胃黏膜,差异有统计学意义(P<0.05)。胃腺癌组织中CCAR1蛋白的表达与分化程度有关(P<0.05),Par-4蛋白的表达与胃腺癌的分化程度、TNM分期、淋巴结转移有关(P<0.05）。Spearman相关分析显示,胃腺癌组织CCAR1蛋白和Par-4蛋白表达无明显相关性(r=-0.08,P>0.05）。结论: Par-4与CCAR1均参与了胃腺癌的发生和进展过程,但二者在胃腺癌的发生过程中可能受不同机制的调控。

关键词: 胃腺癌, 细胞周期与细胞凋亡调控蛋白1（CCAR1）, 前列腺凋亡反应基因-4(Par-4), 免疫组织化学染色

CLC Number:

R735.2

LV Rong-rong, QI Jie-min, CHEN Long. EXPRESSION AND SIGNIFICANCE OF APOPTOSIS RELATED FACTORS CCAR1 AND PAR-4 IN GASTRIC ADENOCARCINOMA[J]. Journal of Chengde Medical University, 2018, 35(6): 451-454.

吕蓉蓉, 齐洁敏, 陈龙. 凋亡相关因子CCAR1、Par-4在胃腺癌中的表达及临床意义[J]. 承德医学院学报, 2018, 35(6): 451-454.

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