Abstract: Objective To investigate the effect of kiss-1 gene expression and overexpression on the proliferation of rectal cancer cells. Methods Human rectal cancer SW620 cells were cultured and randomly divided into 3 groups: blank control group (untransfected), experimental group (transfected with pIRES2-Kiss-1) and negative control group (transfected with empty pIRES2 vector). Immunofluorescence staining and immunocytochemical staining were used to observe the expression of kiss-1 in SW620 cells of rectal cancer. Western blot analysis was employed to examine kiss-1 expression in SW620 cells transfected with piRES2-kiss-1 gene. CCK-8 and MTT assays were used to detect the effect of transfection on SW620 cell proliferation. Results The immune recombinant plasmid pIRES2-Kiss-1 was successfully transfected into rectal cancer SW620 cells. Compared with the blank control group and the negative control group, the kiss-1 protein was highly expressed in the experimental group, and the difference was significant (P<0.05). After cell culture for 48h and 72h, the numbers of SW620 cells in the three groups were significantly lower than that in the blank control group and the negative control group (P<0.05). Conclusion Kiss-1 gene can be expressed efficiently and stably in SW620 cells of rectal cancer, which can effectively reduce the proliferation of SW620 cells in rectal cancer.

Key words: rectal cancer SW620 cells, kiss-1 gene, cell proliferation

摘要： 目的 探讨Kiss-1基因表达与过表达对直肠癌细胞SW620增殖的影响。方法 培养人直肠癌SW620细胞,随机将其分为3组：空白对照组（未转染）、实验组（转染pIRES2- Kiss-1）、阴性对照组（转染pIRES2空载体）。采用免疫荧光染色和免疫细胞化学染色观察直肠癌SW620细胞中Kiss-1的表达情况;Western blot检测转染pIRES2-Kiss-1基因后直肠癌SW620细胞中Kiss-1的表达;采用CCK-8法、MTT法检测转染对SW620细胞增殖的影响。结果 免疫重组质粒pIRES2-Kiss-1成功转染至直肠癌SW620细胞,与空白对照组和阴性对照组相比较,实验组中Kiss-1蛋白高表达,差异有显著性（P<0.05）;3组细胞培养48、72h后,与空白对照组和阴性对照组SW620细胞数相比较,实验组明显降低（P<0.05）。结论 Kiss-1基因在直肠癌SW620细胞中能高效、稳定地表达;Kiss-1基因可有效降低直肠癌SW620细胞的增殖。

关键词: 直肠癌SW620 细胞, Kiss-1基因, 细胞增殖