The Diagnosis and Prognostic Significance of miR-326 in Childhood Acute Lymphoblastic Leukemia

Abstract

Abstract: Objective This study aims to investigate the diagnosis and prognostic significance of miR-326 in childhood acute lymphoblastic leukemia. Methods 70 blood samples from childhood ALL patients and 30 blood samples from healthy volunteers were collected, and qRT-PCR was adopted to detect the relative expression level of serum miR-326. The ROC curve was used to analyze the diagnostic ability and prognostic value of miR-326 in childhood ALL. At the same time, Kaplan-Meier was also performed to draw survival curves to evaluate the survival of children with ALL patients with different expression levels of miR-326. Results Compared with the control group, the expression of miR-326 in the serum of childhood ALL patients was significantly down-regulated (P<0.05). The ROC curve showed an AUC value of 0.876 for the area under the curve, indicating that miR-326 has a good diagnostic value; overall survival analysis showed that low expression of miR-326 suggested a poor survival rate, and the ROC curve showed an AUC value of 0.909. Analysis of progression-free survival showed that low expression of miR-326 suggested a poor progression-free survival rate, and the ROC curve showed an AUC value of 0.815. This indicated that low expression of miR-326 is associated with a poor prognosis. Conclusion According to the expression of miR-326, it is obvious to distinguish children with ALL from healthy people. In addition, the low expression of miR-326 indicates a poor prognosis in children with ALL. Furthermore, miR-326 can be used as a diagnostic and prognostic marker for childhood ALL.

Key words: miR-326, childhood acute lymphoblastic leukemia, diagnosis, prognosis

摘要： 目的本研究旨在探讨miR-326在儿童急性淋巴细胞白血病中的诊断及预后意义。方法收集70份儿童ALL患者血液样本和30份健康志愿者的血液样本,采用qRT-PCR检测血清miR-326的相对表达水平。采用ROC曲线分析miR-326在儿童ALL中的诊断能力和预后价值,并采用Kaplan-Meier绘制生存曲线,评估miR-326表达水平不同的儿童ALL患者的生存情况。结果与对照组相比,miR-326在儿童ALL患者血清中的表达明显下调（P<0.05）。ROC曲线显示曲线下面积AUC值为0.876,表明miR-326有较好的诊断价值;总生存分析显示,低表达的miR-326提示较差的生存率,ROC曲线显示AUC值为0.909。无进展生存分析显示,低表达的miR-326提示较差的无进展生存率,ROC曲线显示AUC值为0.815。表明miR-326低表达与较差的预后相关。结论根据miR-326的表达情况可以很明显的将儿童ALL患者与健康人群进行区分。此外,miR-326低表达提示儿童ALL患者预后不良,即miR-326可作为儿童ALL的诊断及预后标志物。

关键词: miR-326, 儿童急性淋巴细胞白血病, 诊断, 预后

CLC Number:

R733.71

ZHUO Yan-xia, SUN Ming-chang, ZHANG Yong-zhuo. The Diagnosis and Prognostic Significance of miR-326 in Childhood Acute Lymphoblastic Leukemia[J]. Journal of Chengde Medical University, 2021, 38(1): 9-12.

卓艳霞, 孙明昌, 张永卓. 儿童急性淋巴细胞白血病中miR-326的表达及临床意义[J]. 承德医学院学报, 2021, 38(1): 9-12.

References

[1] Pui CH, Yang JJ, Bhakta N, et al.Global efforts toward the cure of childhood acute lymphoblasticleukaemia[J]. Lancet Child Adolesc Health, 2018, 2(6): 440-454.
[2] Mezei G, Sudan M, Izraeli S, et al.Epidemiology of childhood leukemia in the presence and absence of Down syndrome[J]. Cancer Epidemiol, 2014, 38(5): 479-489.
[3] Vasudevan S, Tong Y, Steitz JA.Switching from repression to activation: microRNAs can up-regulate translation[J]. Science, 2007, 318(5858): 1931-1934.
[4] Turunen TA, Roberts TC, Laitinen P, et al.Changes in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress[J]. Sci Rep, 2019, 9(1): 10332.
[5] Sun C, Huang C, Li S, et al.Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development[J]. Oncotarget, 2016, 7(7): 8341-8359.
[6] Hong CC, Chen PS, Chiou J, et al.miR326 maturation is crucial for VEGF-C-driven cortactin expression and esophageal cancer progression[J]. Cancer Res, 2014, 74(21): 6280-6290.
[7] Ji S, Zhang B, Kong Y, et al.miR-326 Inhibits Gastric Cancer Cell Growth Through Downregulating NOB1[J]. Oncol Res, 2017, 25(6): 853-861.
[8] Moya L, Meijer J, Schubert S, et al.Assessment of miR-98-5p, miR-152-3p, miR-326 and miR-4289 Expression as Biomarker for Prostate Cancer Diagnosis[J]. Int J Mol Sci, 2019, 20(5): 1154.
[9] Ward E, DeSantis C, Robbins A, et al. Childhood and adolescent cancer statistics, 2014[J]. CA Cancer J Clin, 2014, 64(2): 83-103.
[10] Pui CH, Evans WE.A 50-year journey to cure childhood acute lymphoblastic leukemia[J]. Semin Hematol, 2013, 50(3): 185-196.
[11] Kato M, Manabe A.Treatment and biology of pediatric acute lymphoblastic leukemia[J]. Pediatr Int, 2018, 60(1): 4-12.
[12] Li Y, Gao Y, Xu Y, et al.Down-regulation of miR-326 is associated with poor prognosis and promotes growth and metastasis by targeting FSCN1 in gastric cancer[J]. Growth Factors, 2015, 33(4): 267-274.
[13] Correction: miR326 Maturation Is Crucial for VEGF-C-Driven Cortactin Expression and Esophageal Cancer Progression[J]. Cancer Res, 2016, 76(1): 182.
[14] Wang S, Lu S, Geng S, et al.Expression and clinical significance of microRNA-326 in human glioma miR-326 expression in glioma[J]. Med Oncol, 2013, 30(1): 373.
[15] Aberuyi N, Rahgozar S, Ghodousi ES, et al.Drug Resistance Biomarkers and Their Clinical Applications in Childhood Acute Lymphoblastic Leukemia[J]. Front Oncol, 2019, 9: 1496.