EFFECTS OF DMY ON MICRORNA373 EXPRESSION IN CHORIOCARCINOMA JAR CELLS

Journal of Chengde Medical University ›› 2017, Vol. 34 ›› Issue (4): 278-280.

EFFECTS OF DMY ON MICRORNA373 EXPRESSION IN CHORIOCARCINOMA JAR CELLS

Institute of Oncology, Chengde Medical College, Hebei Chengde 067000, China

Received:2016-09-30 Online:2017-08-10 Published:2021-11-26

二氢杨梅素对绒癌JAR细胞MicroRNA373表达的影响

王琳¹, 许倩¹, 刘镭¹, 李玉红^1,△, 朱佳蒙², 范兴源²

1.承德医学院肿瘤研究所,河北承德 067000;
2.承德医学院2012级临床本科

Abstract

Abstract: Objective: To investigate the effects of dihydromyricetin (DMY) on MicroRNA373 expression in choriocarcinoma JAR cells. Methods: JAR cells in logarithmic growth phase were treated with different concentrations of DMY (0μg/ml, 60μg/ml, 80μg/ml, 100μg/ml) for 24 hours, then the MicroRNA373 mRNA expression in JAR cells were detected by real time fluorescence quantitative PCR. Results: The MicroRNA373 expression of JAR cells in 3 DMY groups was all obviously higher than blank control group. Moreover, the MicroRNA373 expression gradually increased with increasing of DMY concentration (P<0.05). Conclusions: DMY can increase the MicroRNA373 expression in choriocarcinoma JAR cells in concentration dependent manner.

Key words: Dihydromyricetin (DMY), MicroRNA373, Real time fluorescence quantitative PCR, Choriocarcinoma

摘要： 目的: 探讨二氢杨梅素（DMY）对绒癌JAR细胞MicroRNA373表达的影响。方法: 对数生长期绒癌JAR细胞随机分组,DMY终浓度分别为0μg/ml、60μg/ml、80μg/ml、100μg/ml作用24h,采用实时荧光定量PCR法检测JAR细胞MicroRNA373 mRNA的表达水平。结果: DMY组JAR细胞MicroRNA373的表达均明显高于空白对照组;并且,随着DMY浓度的增加,JAR细胞MicroRNA373的表达逐渐升高（P<0.05）。结论: DMY可升高绒癌JAR细胞MicroRNA373的表达,且呈一定的浓度依赖性。

关键词: DMY, MicroRNA373, 实时荧光定量PCR, 绒毛膜上皮癌

CLC Number:

R737.33

WANG Lin, XU Qian, LIU Lei, et al. EFFECTS OF DMY ON MICRORNA373 EXPRESSION IN CHORIOCARCINOMA JAR CELLS[J]. Journal of Chengde Medical University, 2017, 34(4): 278-280.

王琳, 许倩, 刘镭, 李玉红, 朱佳蒙, 范兴源. 二氢杨梅素对绒癌JAR细胞MicroRNA373表达的影响[J]. 承德医学院学报, 2017, 34(4): 278-280.

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