Objective To investigate the sensitization effect of Ailanthone (AIL) on cisplatin-resistant A549/DDP cells of non-small cell lung cancer. Methods The effects of AIL on the viability of A549 and A549/DDP cells were detected by MTT. Analysis of the combined drug use index(CI) of Ailanone and cisplatin using Chou-Talalay. Cell cycle and apoptosis were detected by flow cytometry, and the expression of apoptosis-related proteins were detected by Western blotting. Results AIL (0.6μmol/L) and DDP (50μg/mL) had synergistic effect. Cell cycle arrest and apoptosis rate increased in G1 phase. The protein expression of caspase-3 and bcl-2 decreased, but the protein expression of Cleaved Caspase3, Bax, CDK4 and CyclinD1 increased. Conclusion AIL enhanced the sensitivity of DDP to A549/DDP, and increased the growth inhibition and apoptosis of DDP on A549/DDP.

Objective: To investigate the expression and significance of Fas-associated death domain (FADD) protein and Caspase-7 protein in gastric adenocarcinoma Methods: The expression of FADD protein and Caspase-7 protein in 60 cases of gastric adenocarcinoma and 50 cases of normal gastric mucosa were detected by immunohistochemical SP method. The relationships between FADD protein, Caspase-7 protein and clinicopathological features of gastric adenocarcinoma, as well as the correlations between FADD protein and Caspase-7 protein in gastric adenocarcinoman were analyzed. Results: The positive products of FADD protein and Caspase-7 protein were all brownish yellow granules, mainly located in the cytoplasm. The positive expression rate of FADD protein and Caspase-7 protein in gastric adenocarcinoma were significantly lower than that in normal gastric mucosa tissues (38.3% vs 60.0%, 46.7% vs 68.0%, P<0.05). In gastric adenocarcinoma, the FADD protein and Caspase-7 protein expression were not correlated with age and gender (P>0.05); but related to the differentiation, TNM stage, lymph node metastasis and infiltration depth (P<0.05). Spearman correlation analysis showed that there had positive correlation between expression of FADD protein and caspase-7 protein in gastric adenocarcinoma (r=0.362, P<0.05). Conclusions: Low expression of FADD protein and Caspase-7 protein may play a synergistic role in the development of gastric adenocarcinoma.

Objective: To investigate the expression and significance of cell division cycle and apoptosis regulator protein1 (CCAR1) and prostate apoptosis response-4 gene (Par-4) protein in gastric adenocarcinoma. Methods: SP immunohistochemical staining was used to detect the expression of CCAR1 and Par-4 in 60 gastric adenocarcinoma tissues and 40 paracancerous normal gastric mucosa tissues. The relationships between CCAR1, Par-4 and clinicopathological parameters of gastric adenocarcinoma, as well as the correlation of CCAR1 and Par-4 in gastric adenocarcinoma were analyzed. Results: The positive expression rate of CCAR1 protein in gastric adenocarcinoma tissues was significantly higher, while the Par-4 protein was significantly lower than that of normal gastric mucosa (P<0.05). The CCAR1 protein expression in gastric adenocarcinoma was related to differentiation degree (P<0.05); The expression of par-4 protein was related to differentiation degree, TNM stage and lymph node metastasisa (P<0.05). Spearman correlation analysis showed that no correlation between the expression of CCAR1 protein and Par-4 protein in gastric adenocarcinoma (r=-0.08, P>0.05). Conclusions: Par-4 and CCAR1 involve in the occurrence and progression of gastric adenocarcinoma together, but they may be regulated by different mechanisms.