Objective To study the effect of production of Interleukin-10(IL-10) in the B16F10 cells by Ganoderma lucidum polysaccharides. Methods The B16F10 cell was prepared and treated with different concentrations of Ganoderma lucidum. The expression levels of IL-10 were assessed by Western-blot, RT-PCR. Results Under Ganoderma lucidum polysaccharides at different concentrations, RT-PCR revealed that the amount of IL-10 mRNA released by B16F10 melanoma cells treated with 0μg/mL, 0.2μg/mL, 0.8μg/mL, 3.2μg/mL, 12.8μg/mL was 1.03±0.15, 1.15±0.25, 0.92±0.11, 0.86±0.64 and 0.75±0.12, respectively. Compared with the 0μg/mL Ganoderma lucidum polysaccharide group, the other groups showed statistical significance except the 0.2μg/mL Ganoderma lucidum polysaccharide group, P<0.05. Western-blot revealed that the amount of IL-10 produced by B16F10 melanoma cell treated with 0μg/mL,0.2 μg/mL, 0.8μg/mL, 3.2μg/mL, 12.8μg/mL was 1.47±0.43, 1.58±0.31, 0.93±0.12, 0.89±0.26 and 0.40±0.29, respectively. Compared with the 0μg/mL Ganoderma lucidum polysaccharide group, the 12.8μg/mL Ganoderma lucidum polysaccharide group showed statistical significance, P<0.05. Conclusion The production of IL-10 in the B16F10 cells was inhibited by Ganoderma lucidum polysaccharides.

Objective This study was conducted to determine the potential of lentinan in protection against methicillin-resistant S. aureus (MRSA) skin infection. Methods Bacterial burden determination and Immunohistochemistry analysis were used to assess the effect of lentinan on antimicrobial infection using a Staphylococcus aureus-infected skin injection model in mice by inoculated with 30μl of 5×10 ⁷ CFU MRSA, and the polymerase chain reaction (RT-PCR) was used to test the effects of lentinan on mRNA expression of IL-33 and chemokine KC at skin abscess in mice. Results Skin wound sections from lentinan -treated in mice was significantly milder than that in control group. Bacterial burden determination and immunohistochemistry analysis showed that significantly decrease bacterial burden and significant increase in CD11b+Gr1+ neutrophils recruitment infiltration, was noted in infectious wounds from lentinan -treated mice. At the same time, mRNA expression of IL-33 and chemokine KC was promptly increased in cutaneous wound tissues when challenged by lentinan. Conclusion Lentinan plays an essential effect of anti-MRSA skin infection in mice.

Objective: To investigate the effects of Ganoderma lucidum polysaccharides (GL-PS) on secretion of vascular endothelial growth factor (VEGF) in B16F10 melanoma cells. Methods: B16F10 melanoma cells were treated with or without different concentrations of GL-PS for 48h, then the expression of VEGF protein and mRNA was respectively detected by Western-blot and RT-PCR. Results: Compared with control group, the expression of VEGF protein and mRNA in B16F10 melanoma cells decreased obviously after the B16F10 melanoma cells were treated with different concentrations of GL-PS (0.8μg/ml, 3.2μg/ml, 12.8μg/ml) for 48h (P<0.05). Conclusions: GL-PS may play a role in inhibiting tumor by inhibiting secretion VEGF in B16F10 melanoma cells.