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Huaier Inducing Apoptosis Through PI3K/AKT Signaling Pathway in Colorectal Cancer
WEN Lu-wen, FENG Qi, HUO Ming-yi, LIU Xin, CHENG Yu, ZHENG Ji-ning
Objective To investigate whether huaier can induce apoptosis of colorectal cancer (CRC) cells and its possible mechanism.
Methods CCK8 assays detected the viability of HCT8 and HT29 cells with different concentration of huaier. Flow cytometry detected the apoptosis of HCT8 and HT29 cells, and the gene expression lever of P85α mRNA was detected by RT-qPCR assays. Western blot assays detected the expression lever of pathway protein P85α, p-AKT and AKT, apoptosis protein BAX, BCL-2, Cleaved caspase-3 and Cleaved caspase-9, and proliferating protein CDK4 and CyclinD1 on HCT8 and HT29 cells with different concentration of huaier.
Results Huaier could reduce the viability of HCT8 and HT29 cells, and the viability were decreasing with the increasing concentration. The apoptosis rates of HCT8 and HT29 cells with different concentration of huaier were increased observably. It showed that huaier could increase the apoptosis rates of HCT8 and HT29 cells, and the apoptosis rates were increasing with the increasing concentration. The expression lever of P85α mRNA in HCT8 and HT29 cells were decreased observably. It showed that huaier could decrease the expression lever of P85α mRNA, and the expression lever of P85α mRNA were decreasing with the increasing concentration. Western blot assays showed that huaier can inhibit the expression lever of P85α, p-AKT/AKT, inhibit the expression of BCL-2, CDK4 and CyclinD1, and promote the expression lever of BAX, Cleaved caspase-3 and Cleaved caspase-9.
Conclusion Huaier can inhibiting the proliferation and inducing the apoptosisof HCT8 and HT29 cells, and its mechanism may be related with PI3K/AKT signaling pathway.
2023, 40 (6):
451-455.
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