Objective To investigate the value of combined detection of carboxylpeptidyme X-M14 family member 1 (CPXM1), laminin subunit β3 (LAMβ3) and homeogenin-like protein 2 (FMNL2) in predicting postoperative recurrence in gastric cancer patients. Methods A total of 107 patients with gastric cancer admitted to the Fifth People's Hospital of Jiaozuo from January 2021 to April 2023 were selected as research objects, patients were divided into a recurrence group (n=24) and a non-recurrence group (n=83) according to their recurrence within 1 year after surgery. And different pathological features and cancer tissue levels of CPXM1, LAMβ3 and FMNL2 were compared between the two groups and their correlation was analyzed, the influencing factors of the gastric cancer patients' postoperative recurrence and their predictive value of the patients' postoperative recurrence were analyzed. Results In the recurrent group, the proportions of T3~T4 depth of invasion, poorly differentiated tumors, and lymph node metastasis, as well as the levels of LAMβ3 mRNA and FMNL2 mRNA in cancer tissues, were all higher than those in the non-recurrent group. Meanwhile, the level of CPXM1 mRNA was lower than that in the non-recurrent group (P<0.05). The level of CPXM1 mRNA in cancer tissues was negatively correlated with TNM staging, lymph node metastasis, and depth of invasion, and positively correlated with the degree of differentiation. Both LAMβ3 and FMNL2 in cancer tissues were positively correlated with TNM staging, lymph node metastasis, and depth of invasion, and negatively correlated with the degree of differentiation (P<0.05). TNM stage III, lymph node metastasis, depth of invasion, LAMβ3, and FMNL2 in cancer tissues were risk factors for postoperative recurrence in patients, while the degree of differentiation and CPXM1 mRNA in cancer tissues were protective factors (P<0.05). The combined CPXM1, LAMβ3 and FMNL2 levels predicted postoperative recurrence of patients with AUC of 0.811, which was higher than that detected by each index alone (P<0.05). Conclusion The changes of CPXM1, LAMβ3 and FMNL2 levels in cancer tissue can provide reference data for the prediction of postoperative recurrence of gastric cancer, and the combined detection has higher clinical application value.