Objective To study the CD68 and VEGF expression in skin lesion tissue and their functions in pathogenesis of the erythema nodosum. Methods The expression of CD68 and VEGF in the skin lesions of 49 cases of erythema nodosum patients (disease group) and 10 cases of normal human (control group) were detected by MaxVisionTM immunohistochemical staining method. Results Compared with the normal control group(60.00%), the positive expression rate of CD68 in disease group(95.92%) was increased significantly, with statistical differences between two groups(P<0.01). Compared with the normal control group(60.00%), the positive expression rate of VEGF in disease group(95.92%) was increased significantly, with statistical differences between two groups (P<0.01). Conclusion Higher expression of CD68 and VEGF in skin lesions was concluded that active inflammatory cells and injury of endothelial cell were participated in pathogenesis of erythema nodosum.

Objective: To study the changes and significance of CD4 and CD8 expression in skin lesions of patients with erythema nodosum. Methods: MaxVision ^TM immunohistochemical stain was used to detect the expression of CD4 and CD8 in skin lesions of 49 cases of erythema nodosum patients (disease group) and 10 cases of normal skin (control group). Results: The CD4 positive rate of disease group was 100.00%, which was obviously higher than control group (P<0.01). There was no significant difference of positive rate of CD8 expression between disease group and control group (P>0.05). Conclusions: There is an imbalance of T cells subgroups in skin lesions of erythema nodosum patients, which suggests that the cellular immune response mediated by CD4 ⁺ T cells plays an important role in the pathogenesis of erythema nodosum.