Objective To check the differential expression of apoptosis-related factor Bid in adenocarcinoma of stomach tissue an and analyze its clinical significance. Methods 60 cases of adenocarcinoma of stomach tissues were the experimental group, and 55 cases of normal gastric mucosal epithelium tissue were the control group. The expression of Bid protein and mRNA was comprehensively detected by combined immunohistochemical SP method, Western blot and qRT-PCR technology. Results Compared with normal gastric mucosal epithelium tissue, the positive expression level of Bid protein and the relative expression level of mRNA in adenocarcinoma of stomach tissue are reduced, and the differences are statistically significant (P<0.05). In protein level and gene level, the Bid molecule expression level in adenocarcinoma of stomach tissues was related to the degree of differentiation, lymph node metastasis, clinical TNM stage and infiltration depth(P<0.05). Conclusion The apoptosis-related factor Bid plays a vital role in the occurrence and development of adenocarcinoma of stomach, and it may become a new target for the treatment of adenocarcinoma of stomach.

Objective: To investigate the expression and significance of Fas-associated death domain (FADD) protein and Caspase-7 protein in gastric adenocarcinoma Methods: The expression of FADD protein and Caspase-7 protein in 60 cases of gastric adenocarcinoma and 50 cases of normal gastric mucosa were detected by immunohistochemical SP method. The relationships between FADD protein, Caspase-7 protein and clinicopathological features of gastric adenocarcinoma, as well as the correlations between FADD protein and Caspase-7 protein in gastric adenocarcinoman were analyzed. Results: The positive products of FADD protein and Caspase-7 protein were all brownish yellow granules, mainly located in the cytoplasm. The positive expression rate of FADD protein and Caspase-7 protein in gastric adenocarcinoma were significantly lower than that in normal gastric mucosa tissues (38.3% vs 60.0%, 46.7% vs 68.0%, P<0.05). In gastric adenocarcinoma, the FADD protein and Caspase-7 protein expression were not correlated with age and gender (P>0.05); but related to the differentiation, TNM stage, lymph node metastasis and infiltration depth (P<0.05). Spearman correlation analysis showed that there had positive correlation between expression of FADD protein and caspase-7 protein in gastric adenocarcinoma (r=0.362, P<0.05). Conclusions: Low expression of FADD protein and Caspase-7 protein may play a synergistic role in the development of gastric adenocarcinoma.