Objective To investigate the effects of annexinA7 (ANXA7) overexpression on the proliferation and apoptosis of human gastric cancer MGC-803 cells. Methods Human gastric cancer MGC-803 cells were divided into ANXA7 overexpression group and negative control group. PcDNA3.1-ANXA7 recombinant plasmid and empty plasmid were transfected into ANXA7 overexpression group and negative control group respectively by liposome transfection. The overexpression of ANXA7 was detected by Western blot. CCK-8 assay was used to detect the proliferation ability of cells in each group. Cell apoptosis rate was detected by flow cytometry, and the expressions of PCNA, CyclinD1, Casepase-3 and Cleaved Casepase-3 were detected by Western blot. Results Compared with the negative control group, the proliferation ability of MGC-803 cells in the ANXA7 overexpression group was significantly enhanced, and the expression of PCNA and CyclinD1 were both increased (P<0.05). Apoptosis rate and Cleaved casepase-3 expression were decreased (P<0.05). The expression of Casepase-3 was not statistically significant (P>0.05). Conclusion The overexpression of ANXA7 can promote the proliferation and inhibit the apoptosis of human gastric cancer MGC-803 cells.