Objective To explore the anti-inflammatory mechanism of loranthus parasiticus based on network pharmacology and molecular docking technology. Methods Active components and targets of loranthus parasiticus were gathered by TCMSP database and screened inflammatory targets by GeneCards, TTD and OMIM database. A “compound-target-disease” network was constructed with Cytoscape3.7.1. Protein interaction networks were mapped using String database and Clusterprofiler R Package was applied to enrich gene ontology GO and KEGG pathways of loranthus parasiticus intervention in inflammation. Molecular docking verification was carried out in AutoDockTools and AutoDockVina, and the results were visualized by PyMOL software. Results Forty-six active components and 418 potential targets of loranthus parasiticus, 696 targets of inflammation and 71 common targets of loranthus parasiticus and inflammation were collected. GO enrichment analysis and KEGG pathway enrichment analysis screened out 1703 items and 139 signaling pathways (P<0.05), mainly involving lipid and atherosclerosis, AGE-RAGE signaling pathway, TNF-α, IL-17 signaling pathway, Toll-like receptor signaling pathway, etc. The molecular docking results showed that quercetin had the strongest binding ability to AKT1. Conclusion Loranthus parasiticus may regulate multiple signaling pathways including IL-6, IL-1β, RelA, MAPK1 and AKT1 through quercetin, oleanolic acid and guajavarin to exert anti-inflammatory effects, providing reference for future studies.

Objective To establish an HPLC method for measuring the content of Chaihu saponina, c and d, which is used to compare the differences in the content of wild beichaihu in different origin cultivation areas of Beichaihu and Chengde City, Hebei Province. Methods The content of Chaihu saponin a, c and d in 27 batches of different samples of Beichaihu was determined by high-efficiency liquid chromatography, and the correlationship was analysed. Results The quality of cultivated chaihu was significantly higher than that of wild Chaihu, the highest level of Chaihu saponin a was in Beichahu, Gansu Kang County, and the highest content of Chaihu saponin d was in Beichaihu of Gansu Province, most of which were not detected by the wild beihu saponin c, and the cultivation of beihu saponin c was detected. The color and saponin content of Chaihugen were significantly correlated, the yellow root saponin a, d content was the highest, the black root content was second, and the root color had little effect on the content of Chaihu saponin c. Conclusion The content of artificial cultivation of Beichaihu saponina, c and d is higher than that of wild Chaihu, and it is found that the content of beichaihugen color and saponin content are significantly correlated, which provides theoretical basis for the establishment and evaluation of Chaihu resource utilization and quality analysis methods of medicinal herbs.

Objective To explore the expression relationship and significance of intramedullary IL-4 and CD34 in rabbit VX2 bone tumors. Methods Thirty Japanese white rabbits were divided into experimental group and control group with 15 rabbits each by random number method. In the experimental group, the left tibia was implanted with VX2 tumor tissue through the tibial plateau to make a bone tumor model. The control group adopted the same operation method but did not implant the VX2 tumor tissue. Closed biopsy was used to extract the bone marrow of the left proximal tibia 7 days and 14 days after operation, and the levels of IL-4 and CD34 in all specimens were detected by enzyme-linked immunosorbent assay (Elisa). At the same time, 14 days after operation, tibia tissue was taken for histopathological examination after execution. Results 30 rabbits were successfully modeled and included in the study. On the 7th day after operation, the intramedullary IL-4 and CD34 contents of rabbits were significantly higher than those before operation, and the difference was statistically significant (P<0.05). On the 14th day after the operation, the IL-4 and CD34 levels were significantly higher than those on the 7th day after the operation. The difference was statistically significant (P<0.05). The pathology showed that the tumor tissue was in the tibial marrow cavity in the bone tumor model group 14 days after the tumor was implanted. Significant tumour growth with tumour cells visible in the bone cortex. Conclusion Within 14 days of modeling rabbit VX2 bone tumor animal model, as the tumor proliferates rapidly, the body's anti-tumor immune function is gradually inhibited, which is manifested by the increased expression of IL-4 and CD34.