Objective To analyze the diagnostic value of carotid cerebral integrated ultrasound (Transcranial Doppler and carotid artery ultrasound) in detecting the degree of intracranial external arterial stenosis in the diagnosis of coronary artery stenosis. Methods A total of 186 patients who underwent coronary angiography in the Affiliated Hospital of Chengde Medical University from January 2019 to December 2020 were selected as the study subjects, and were divided into two groups: the coronary heart disease group (98 cases) and the non coronary heart disease group (88 cases). The patients in the coronary heart disease group were then divided into a single vessel disease group (32 cases) and a multi vessel disease group (66 cases) based on the main coronary artery disease and the number of affected branches. Transcranial Doppler (TCD) and carotid artery ultrasound were performed in both groups to measure carotid intima-media thickness (IMT), plaque thickness, and number, and calculate plaque scores. In addition, carotid and intracranial artery stenosis were counted in both groups, and the risk factors affecting the degree of coronary atherosclerosis were analyzed by logistic regression analysis. Results The body mass index, smoking, hypertension, hyperlipidemia, and serum hs-CRP levels in the coronary heart disease group were significantly higher than those in the non coronary heart disease group. Carotid artery ultrasound examination results showed that the IMT and plaque score in patients with coronary heart disease were higher than those in patients without coronary heart disease. The IMT and plaque score in the subgroup of multi vessel disease in the coronary heart disease group were higher than those in the single vessel disease group. The severity of carotid stenosis in patients with coronary heart disease was significantly higher than those in the non coronary heart disease group. The results of TCD examination showed that the severity of intracranial artery stenosis in patients with coronary heart disease was significantly higher than that in patients without coronary heart disease. The more severe the stenosis of intracranial and internal carotid arteries was, the higher the degree of coronary artery disease was, and the more branches of diseased vessels were. Multivariate logistic regression analysis showed that hypertension, hyperlipidemia, internal carotid artery stenosis , intracranial artery stenosis, body mass index, hs-CRP are independent risk factors that affecting the degree of coronary atherosclerosis. Conclusion Combined carotid and cerebral ultrasound can effectively diagnose the degree of intracranial and external vascular stenosis, and there is a significant positive correlation between the degree of intracranial and external arterial stenosis and the degree of coronary artery stenosis. The degree of intracranial and carotid artery stenosis are independent risk factors that affect the degree of coronary artery stenosis.

Objective To study the mechanism of matrine inhibiting human colon cancer cells through the IL-6/STAT3 signaling pathway. MethodHuman colon cancer cell HCT-116 was interfered with matrine, cell proliferation assay CCK-8 was used to detect cell proliferation, and the protein expression level of inflammatory factor IL-6/STAT3 gene and the protein expression level of metastatic power of colon cancer cell E-CAD gene were detected by Western blot assay. Results The cell proliferation test showed that the cell proliferation of 2mg/mL, 3mg/mL and 4mg/mL matrine groups was significantly lower than that of the control group. Western blot results showed that the matrine group had IL-6 / STAT3 protein compared with the control The expression level was significantly decreased (P<0.05), and the expression level of E-Cad protein was significantly increased(P<0.05). Conclusion Matrine can reduce the inflammatory response of human colon cancer cell HCT-116 by inhibiting IL-6/STAT3 signaling pathway, and further inhibit the proliferation and invasiveness of human colon cancer cell HCT-116, so as to achieve anti-tumor effect.