Objective To investigate whether huaier can induce apoptosis of colorectal cancer (CRC) cells and its possible mechanism. Methods CCK8 assays detected the viability of HCT8 and HT29 cells with different concentration of huaier. Flow cytometry detected the apoptosis of HCT8 and HT29 cells, and the gene expression lever of P85α mRNA was detected by RT-qPCR assays. Western blot assays detected the expression lever of pathway protein P85α, p-AKT and AKT, apoptosis protein BAX, BCL-2, Cleaved caspase-3 and Cleaved caspase-9, and proliferating protein CDK4 and CyclinD1 on HCT8 and HT29 cells with different concentration of huaier. Results Huaier could reduce the viability of HCT8 and HT29 cells, and the viability were decreasing with the increasing concentration. The apoptosis rates of HCT8 and HT29 cells with different concentration of huaier were increased observably. It showed that huaier could increase the apoptosis rates of HCT8 and HT29 cells, and the apoptosis rates were increasing with the increasing concentration. The expression lever of P85α mRNA in HCT8 and HT29 cells were decreased observably. It showed that huaier could decrease the expression lever of P85α mRNA, and the expression lever of P85α mRNA were decreasing with the increasing concentration. Western blot assays showed that huaier can inhibit the expression lever of P85α, p-AKT/AKT, inhibit the expression of BCL-2, CDK4 and CyclinD1, and promote the expression lever of BAX, Cleaved caspase-3 and Cleaved caspase-9. Conclusion Huaier can inhibiting the proliferation and inducing the apoptosisof HCT8 and HT29 cells, and its mechanism may be related with PI3K/AKT signaling pathway.

Objective To explore the risk factors of postoperative recurrence of benign meningioma. Methods A total of 241 cases of postoperative patients with benign meningiomas in Affiliated Hospital of Chengde Medical University were selected as the research object (using a retrospective method), the selection time is from January 2018 to March 2021, and all the patients' clinical basic data were sorted and analyzed. According to whether the recurrence within 1 year after surgery, patients were divided into no recurrence group (212 cases) and recurrence group (29 cases). Single factor analysis was applied to analyze the clinical data of the two groups, and multivariate Logistic regression analysis was applied to analyze the statistically significant factors and screen out the independent risk factors for postoperative recurrence of benign meningioma. Results Among the postoperative patients with benign meningiomas, the proportion of patients with tumor site (cerebral falx or venous sinus), maximum tumor diameter>5cm, partial tumor resection, unclear tumor boundary, histopathological endothelial type/fibroblast and Ki-67 expression≥8% in the recurrence group was higher than that in the non-recurrence group, and the difference was statistically significant (P<0.05). There was no statistically significant difference in gender, age, tumor shape, peritumoral edema, meningeal tail sign, FASN expression and PCNA expression between the two groups (P>0.05). Tumor location (cerebral falx or venous sinus), tumor maximum diameter>5cm, partial tumor resection, unclear tumor boundary, histopathological endothelial type/fibroblast and Ki-67 expression≥8% were all risk factors for recurrence of benign meningioma postoperative patients. The differences were statistically significant (P<0.05). Conclusion Tumor site (cerebral falx or venous sinus), maximum tumor diameter>5cm, partial tumor resection, unclear tumor boundary, histopathological endothelial type/fibroblast and Ki-67 expression≥8% are all risk factors for recurrence of patients with benign meningioma after operation. Therefore, corresponding clinical measures can be taken to prevent and treat patients with benign meningioma after operation to improve their prognosis and reduce recurrence rate.

Objective: To investigate the expression and clinical significance of adisintegrin and metalloprotease-17 (ADAM17) and epidermal growth factor receptor(EGFR) in endometrial carcinoma. Methods: Immunohistochemical staining was used to detect the ADAM17 and EGFR expression in 20 cases of normal endometrium, 20 cases of atypical hyperplasia endometrium and 50 cases of endometrial carcinoma. Results: The positive expression rate of ADAM17 and EGFR in endometrial carcinoma were all obviously higher than normal endometrium and atypical hyperplasia endometrium (P<0.01). In endometrial carcinoma, the expression of ADAM17 and EGFR were related to muscular infiltration depth and lymph node metastasis (P<0.05), but not related to age and whether menopause (P>0.05). In endometrial carcinoma, the expression of ADAM17 was positively correlated to EGFR (r=0.691, P<0.01). Conclusions: ADAM17 and EGFR may play important role in development and metastasis of endometrial carcinoma.