Objective To study the mechanism of matrine inhibiting human colon cancer cells through the IL-6/STAT3 signaling pathway. MethodHuman colon cancer cell HCT-116 was interfered with matrine, cell proliferation assay CCK-8 was used to detect cell proliferation, and the protein expression level of inflammatory factor IL-6/STAT3 gene and the protein expression level of metastatic power of colon cancer cell E-CAD gene were detected by Western blot assay. Results The cell proliferation test showed that the cell proliferation of 2mg/mL, 3mg/mL and 4mg/mL matrine groups was significantly lower than that of the control group. Western blot results showed that the matrine group had IL-6 / STAT3 protein compared with the control The expression level was significantly decreased (P<0.05), and the expression level of E-Cad protein was significantly increased(P<0.05). Conclusion Matrine can reduce the inflammatory response of human colon cancer cell HCT-116 by inhibiting IL-6/STAT3 signaling pathway, and further inhibit the proliferation and invasiveness of human colon cancer cell HCT-116, so as to achieve anti-tumor effect.

Objective: To investigate the feasibility of evaluation of the therapeutic effects of interventional therapy on nerve root type cervical disc herniation by infrared thermal imaging technology. Methods: 50 cases of nerve root type cervical disc herniation patients were all treated by plasma radiofrequency, ozone ablation combined collagenolysis. The CASCS score, neck and shoulder temperature by infrared thermal imaging technology of patients before operation and 6 months after operation were recorded. Results: The CASCS score of patients 6 months after operation were obviously higher than that before operation (P<0.01). The neck and shoulder temperature of affected side before, after operation were all higher than healthy side (P<0.01,P<0.05); And the difference value of temperature between healthy side and affected side after operation was obviously lower than that before operation (P<0.01). Correlation analysis showed that the changes of difference value of neck and shoulder temperature before and after operation was negatively correlated to changes of CASCS score (r=-0.614,P<0.01). Conclusions: Infrared thermal imaging technology can be used to evaluate the therapeutic effects of plasma radiofrequency, ozone ablation combined collagenolysis in treatment of nerve root type cervical disc herniation.

Objective: To investigate the efficacy and safety of treating idiopathic trigeminal neuralgia with pulsed radiofrequency combined adriamycin. Methods: 90 idiopathic trigeminal neuralgia patients failed to conservative treatment were randomly divided into adriamycin (ADM) group, pulsed radiofrequency combined adriamycin (PRF+ADM) group and PRF group with 30 patients in each group.The VAS score,PRI score,effective rate and complications of patients in 3 groups were compared. Results: The VAS score, PRI score of patients in PRF+ADM were obviously lower than that of patients in PRF group at each time point after treatment,but the effective rate was obviously higher (P<0.05). The VAS score, PRI score and effective rate had statistical difference between PRF+ADM group and ADM group 1d and 3d after treatment (P<0.05); There had no statistical significance of VAS score, PRI score and effective rate between PRF+ADM group and ADM group at other time point after treatment. There were no obvious complications in 3 groups. Conclusions: Treating idiopathic trigeminal neuralgia with pulsed radiofrequency combined adriamycin can get better short-term and long term effects, as well as higher security, so it is worthy of clinical application.