Objective: To investigate the regulatory effects of matrix stiffness on the elastic modulus and cytoskeleton of macrophages. Methods: Mouse macrophage RAW264.7 were cultured on different stiffnesses of polyacrylamide gels (2kPa and 20kPa). The nano-indenter was used to detect the elastic modulus of macrophages cultured on different matrix stiffness gels, flow cytometry to detect the F-actin content in macrophages. Results: Compared with 2kPa matrigel, elastic modulus and F-actin content of macrophages that cultured on 20kPa matrigel increased obviously (P<0.05). Conclusions: Matrix stiffness can regulate the elastic modulus and cytoskeleton of macrophages, but the mechanism needs to be further studied.

Objective: To analyze the effects of tropomodulin1 (Tmod1) on urine concentration in kidney of mice. Methods: Tubule-specific Tmod1 knockout mice, Tmod1 ^flox/flox/Ksp-cre ⁺ (TFK) and its control mice Tmod1 ^flox/flox/Ksp-cre ^-(TF)were tanken as research object in this study. The urine of mice was collected and the 24-hour urine volume as well as urine osmolality of all the mice were measured at basic state, water deprivation and water loading using metabolic cage system. Results: At basic state, water deprivation and water loading, the 24-hour urine volume of TFK mice was remarkably lower than TF mice, while the urine osmolality of TFK mice was remarkably higher than TF mice (P<0.05). Conclusions: Tmod1 may be involved in the process of urine concentration in mice, but the mechanisms need to be further studied.