Objective:To establish the method for simultaneous determination of rhamnosylvitexin, glucosylvitexin, hyperoside and vitexin content in Hawthorn leaf extract by quantitative analysis of multi-components by single marker. Methods:The relative correction factor of rhamnosylvitexin, hyperoside and vitexin were calculated by using glucosylvitexin as internal standard, and the content determination results were compared with the traditional external standard curve method. Results:The relative correction factor between glucosylvitexin and rhamnosylvitexin, hyperoside, vitexin were respectively 0.963, 1.116 and 1.034. There was no obvious difference about the rhamnosylvitexin, hyperoside and vitexin content between quantitative analysis of multi-components by single marker and traditional external standard curve method. Conclusions:The quantitative analysis of multi-components by single marker in this study can be used in simultaneous determination of 4 components in Hawthorn leaf extract.

Objective: To construct the HPLC fingerprint of Yixintong tablets. Methods: The HPLC was used to construct the fingerprint of 13 batches Yixintong tablets with the condition that the column was ZORBAX SB-C ₁₈ column (5µm, 4.6mm×250mm); the mobile phase was eluted with gradient by 0.1% formic acid-water, acetonitrile and tetrahydrofuran; the flow rate was 1.0ml/min; the detection wavelength was 350nm; the column temperature was 30℃; the sample volume was 10μl. The similarity evaluation software (2004A version) was used for similarity analysis. Results: The HPLC fingerprints of 13 batches Yixintong tablets calibrated 10 common peaks, and the similarity were all higher than 0.99. Conclusions: The method is simple, stable and feasible, and can be used to evaluate the quality of Yixintong tablets.