Objective To investigate the effect of diosgenin on CD4 ⁺CD25 ^-T cells of DBA1/J mice. Methods CD4 ⁺CD25 ^-T cells of DBA1/J mice were separated with immunomagnetic beads. Then, CD4 ⁺CD25 ^-T cell purity was detected by flow cytometry and culture them in vitro. After treated with diosgenin, the concentration of IL-10 was detected by ELISA, the level of transcription factor Foxp3 mRNA was detected by real-time PCR, and the proportion of Treg cells was detected by FCM. Results This study separated CD4 ⁺CD25 ^-T cells of 93.89% purity. Compared with the control group, the concentration of IL-10 in cell supernatant, the Foxp3 mRNA level and percentage of Treg cells were upregulated significantly (P<0.05) after treated with diosgenin for 48h. Conclusion Diosgenin can significantly upregulate the levels of IL-10 and Foxp3 mRNA, the proportion of Treg cells in DBA1/J mice.

Objective: To study the spasticity of rat tail and the changes of dopamine receptor-1 (DRD1) expression in spinal cord after spinal cord injury (SCI). Methods: Adult male Wistar rats were randomly divided into sham operation group and SCI group. The 2 ^nd sacral spinal cord (S ₂) of rats in SCI group were transected totally; And then the tail spasticity score of rats were evaluated 2d, 7d, 14d, 30d and 60d after operation respectively. Immunofluorescence was used to detect the DRD1 expression in gray matter of sacral and coccygeal spinal cord 60d after operation. Results: The tail spasticity score of rats in SCI group 2d, 7d, 14d, 30d and 60d after operation were respectively 0, 1.10±0.28, 1.32±0.85, 2.50±0.53 and 4.15±0.36. DRD1 expression could be observed in dorsal horn (DH), intermediate zone (IMZ) and ventral horn (VH) in gray matter of sacral and coccygeal spinal cord of rats. The DRD1 expression in DH, IMZ and VH in gray matter of spinal cord of rats in SCI group were significantly lower than rats in sham operation group (P<0.05). Conclusions: Decreased expression of DRD1 in spinal cord after SCI may be associated with development of tail spasticity.