Objective: To observe the effects of sericin on Bax expression in rat insulinoma cell (INS-1 cell) injured by streptozotocin (STZ). Methods: INS-1 cells were divided into 3 groups: normal control group, the cells were not treated with any drugs; STZ treatment group, the cells were treated with STZ (10mmol/L) for 24h; sericin protection group, the cells were treated with STZ (10mmol/L) and sericin (300μg/ml) for 24h. CCK8 assay was used to measure the viability of INS-1 cells in each group; Western blot and real-time fluorescence quantitative PCR were used to detect the Bax protein and mRNA expression in INS-1 cells. Results: The viability of INS-1 cells in STZ treatment group was remarkably lower than normal control group, while the Bax protein and mRNA expression were remarkably higher (P<0.05). The viability of INS-1 cells in sericin treatment group was remarkably higher than STZ treatment group, while the Bax protein and mRNA expression were remarkably lower (P<0.05). Conclusions: Sericin has protective effects on INS-1 cells injured by STZ, the mechanisms maybe concerned with it can down regulate Bax expression.

Objective: To observe the effects of hydromorphone combined with flurbiprofen for postoperative analgesia in orthopedic lower extremity surgery. Methods: 90 cases of orthopedic lower extremity surgery patients were randomly divided into SF group, K group and KH group with 30 patients in each group. The patients in 3 groups used sufentanil (SF group: 2μg/kg~3μg/kg), hydromorphone (K group: 0.12mg/kg~0.14mg/kg) and hydromorphone+flurbiprofen (KH group: 0.12mg/kg+50mg) for postoperative analgesia respectively. The press times of patient-controlled intravenous analgesia (PCIA), VAS score and Ramsay score (2h, 6h, 12h, 24h after operation), as well as adverse reactions (skin itch, drowsiness, dizziness, nausea and vomiting, respiratory depression) of patients in 3 groups were compared. Results: The VAS score at each time point after operation, the press times of PCIA, adverse reactions rate of patients in KH group were all obviously lower than SF group and K group (P<0.05). There was no significant difference about the Ramsay score at each time point after operation of the 3 groups (P>0.05). Conclusions: Hydromorphone combined with flurbiprofen for postoperative analgesia in orthopedic lower extremity surgery is a safe and effective postoperative analgesic method, which can obtain better analgesic effects and less adverse reactions.