Objective To investigate the effects of hypoxia on the expression of E-cadherin, Vimentin, MMP-2 and MMP-9 in pancreatic cancer PANC-1 cells. Methods Pancreatic cancer PANC-1 cells were divided into control group and hypoxic group. The hypoxic conditions were 5% CO ₂, 2% O ₂ and 93% N ₂ while the control condition is 5% CO ₂ and 95% O ₂. The expression levels of E-cadherin, Vimentin, MMP-2 and MMP-9 were detected by qRT-PCR and Western blot. Results The E-cadherin protein expression of control group and hypoxia group were 0.859±0.087 and 0.321±0.033 respectively; the Vimentin protein were 0.453±0.051 and 1.128±0.169 respectively; E-cadherin mRNA were 1.125±0.092 and 0.352±0.036 respectively; Vimentin mRNA were1.253±0.130 and 2.875±0.366 respectively. All the differences were statistically significant (P<0.05); The MMP-2 protein and mRNA level in the control and hypoxia groups were 0.254±0.031 and 0.839±0.073, 1.230±0.125 and 4.369±0.368 respectively and the difference was statistically significant (P<0.05); The MMP-9 protein and mRNA in the control and hypoxia groups were 0.268±0.039and 0.733±0.075, 1.452±0.023and 4.136±0.569. The difference was statistically significant (P<0.05). Conclusion Hypoxia can promote the invasion and migration of pancreatic cancer cells by regulating the metastasis-related genes.

Objective: To study the relationships between common digestive diseases and Helicobactor pylori infection in Chengde area. Method: The gastric mucosa specimens of 1036 patients (urease test positive) with chronic gastritis, duodenal ulcer, gastric ulcer, complex ulcer, dyspepsia, gastric carcinoma in Chengde Central Hospital were taken for Helicobactor pylori culture. Results: Among 1036 patients, the Helicobactor pylori were positive in 448 patients and the positive rate was 43.2%. The Helicobactor pylori positive rate in different digestive system diseases in Chengde area from high to low was dyspepsia (100%), duodenal ulcer (69.3%), complex ulcer (68.3%), gastric ulcer (49.4%), gastric carcinoma (29.3%) and chronic gastritis (19.8%). Conclusions: In Chengde area, the common digestive diseases, such as chronic gastritis, dyspepsia, peptic ulcer and gastric cancer are closely related to Helicobactor pylori infection. Correct understanding and treatment ofHelicobacter pylori infection has positive significances for reducing disease burden and improving the life quality of patients.

Objective: To investigate the expression and clinical significance of adisintegrin and metalloprotease-17 (ADAM17) and epidermal growth factor receptor(EGFR) in endometrial carcinoma. Methods: Immunohistochemical staining was used to detect the ADAM17 and EGFR expression in 20 cases of normal endometrium, 20 cases of atypical hyperplasia endometrium and 50 cases of endometrial carcinoma. Results: The positive expression rate of ADAM17 and EGFR in endometrial carcinoma were all obviously higher than normal endometrium and atypical hyperplasia endometrium (P<0.01). In endometrial carcinoma, the expression of ADAM17 and EGFR were related to muscular infiltration depth and lymph node metastasis (P<0.05), but not related to age and whether menopause (P>0.05). In endometrial carcinoma, the expression of ADAM17 was positively correlated to EGFR (r=0.691, P<0.01). Conclusions: ADAM17 and EGFR may play important role in development and metastasis of endometrial carcinoma.