Objective To investigate the relationship between the levels of growth differentiation factor-15 (GDF-15), vascular endothelial growth factor (VEGF), and CXC chemokine receptor 4 (CXCR4) and in-stent restenosis (ISR) in patients with coronary heart disease after percutaneous coronary intervention (PCI). Methods A total of 118 patients with coronary heart disease admitted to Henan Provincial Rongjun Hospital from March 2021 to June 2022 were selected and all received PCI. They were divided into ISR group (24 cases) and non-ISR group (94 cases) according to whether ISR occurred after PCI. Enzyme-linked immunosorbent assay was used to measure GDF-15 and CXCR4, and double antibody sandwich-enzyme-linked immunosorbent assay was used to measure VEGF. The correlation and predictive value of serum GDF-15, VEGF, CXCR4 and ISR in patients with coronary heart disease after PCI were analyzed. Results The number of coronary lesions and the number of stents implanted in the ISR group were higher than those in the non-ISR group, and the length of coronary lesions was longer than that in the non-ISR group. The preoperative Gensini score, left ventricular ejection fraction (LVEF), D-dimer (D-D), fibrinogen (FIB), GDF-15, and VEGF were higher than those in the non-ISR group, while CXCR4 was lower than that in the non-ISR group, and the differences were statistically significant (P<0.05). Increased levels of FIB, GDF-15, VEGF, and decreased levels of CXCR4 were independent risk factors for the development of ISR after PCI, and the differences were statistically significant (P<0.05). After further adjusting for the influence of FIB, GDF-15, VEGF, and CXCR4 remained associated with the development of ISR after PCI. The AUC of the combined prediction of serum GDF-15, VEGF, and CXCR4 for predicting ISR after PCI was significantly greater than that of each individual predictor, and the differences were statistically significant (P<0.05). Conclusion Serum GDF-15, VEGF and CXCR4 levels are significantly correlated with ISR in patients with coronary heart disease after PCI. All of them have predictive value for ISR, and combined detection can provide more reliable data support for clinical practice.

Objective To investigate the effect of the expression level of S100 calcium-binding protein A16 in lung adenocarcinoma (LUAD) tissues on patient prognosis, and the effect of overexpression of S100A16 on the malignant behavior of lung cancer cells. Methods The expression level of S100A16 in lung cancer microarray was detected by immunohistochemistry. According to the expression level of S100A16 in tumor tissues, immunohistochemical scores were performed to statistically analyze the relationship between S100A16 expression and clinicopathological parameters and patient prognosis. The gene expression of S100A16 in lung adenocarcinoma tissues and normal tissues was analyzed by GEPIA database. After S100A16 was knocked down in lung cancer cell line, cell migration and invasion ability were detected by cell scratch assay and Transwell assay, and the proliferation ability of lung cancer cells was detected by Edu assay. Results Immunohistochemical results showed that the expression level of S100A16 was significantly up-regulated in tumour tissues compared to paraneoplastic tissues, and the elevated level of S100A16 expression was associated with lymph node metastasis, tumour stage and distant organ metastasis (P<0.05), but not related to differentiation degree, age, or gender (P>0.05). Survival analysis showed that the higher the level of S100A16 expression in tumour tissue, the worse the prognosis of patients (P<0.001). The vitro experiment showed that inhibition of S100A16 expression suppressed the proliferation and invasive ability of tumour cells (P<0.001). Conclusion The expression level of S100A16 in LADC tumor tissues is elevated, and its expression level is related to clinicopathological parameters. Patients with elevated S100A16 expression tend to have a poorer prognosis. The expression level of S100A16 can regulate the proliferation and invasion of tumor cells.

Objective To analyze the expression of fractalkine (FKN), heart-type fatty acid-binding protein (H-FABP) and D-dimer (D-D) in acute pulmonary embolism and their clinical significance. Methods A total of 43 patients with acute pulmonary embolism admitted to Zhoukou First People's Hospital from October 2020 to October 2021 were selected as the study group, and 50 healthy people who underwent physical examination during the same period were selected as the control group. The levels of serum FKN, H-FABP and D-D in the two groups were measured by double antibody sandwich ELISA and immunoturbidimetry respectively, and their expression and clinical significance were analyzed. Results Compared with low-risk and medium-risk patients in control group and study group, the levels of FKN, H-FABP and D-D in high-risk patients were higher (P<0.05), FKN, H-FABP and D-D were positively correlated with the risk grade of acute pulmonary embolism. FKN and H-FABP were positively correlated in patients with acute pulmonary embolism (r=0.503, P=0.010). There was positive correlation between FKN and D-D (r=0.682, P=0.001). There was positive correlation between H-FABP and D-D (r=0.755, P=0.001). ROC curve analysis showed that FKN, H-FABP and D-D (AUC =0.651,0.692,0.740) combined (AUC =0.844) had higher diagnostic value for acute pulmonary embolism (P<0.05). Conclusion The occurrence and pathological progress of acute pulmonary embolism may be related to the abnormal expression of serum FKN, H-FABP and D-D, and the combination of the three has important significance for the early diagnosis of acute pulmonary embolism.