Objective In order to investigate the application of anti-Dsg1and anti-Dsg3 antibody in diagnosis and treatment of patients, the level and changes of anti-Dsg1and anti-Dsg3 antibody in 18 patients with pemphigus and the relationship between the antibody index and the severity of the disease were analyzed. Methods 18 patients were diagnosed for pemphigus by direct immunofluorescence and pathology, and the patient's pathogenetic condition was assessed by ABSIS standard.The level of Dsg1 and Dsg3 of patients and nomals were detected by enzyme-linked immunosorbent assay(ELISA). Statistics was performed to analyze the relationship and significance of each index at last. Results Among the 18 patients, 13 were Dsg1 positive and 10 Dsg3 positive . The index of Dsg1 and Dsg3 after treatment were decreased than before, and the difference was statistically significant (P<0.05). Of the 20 nomals, 2 were Dsg1 positive and 1 was Dsg3 positive. The sensitivity of Dsg1 and Dsg3 antibody detection was 72.22% and 55.56% respectively, and the specificity was 90% and 95% respectively. The index of Dsg antibody was correlated with the ABSIS score (ALL P<0.05). Conclusion The Dsg antibody level of patients with pemphigus was correlated with the severity of the disease with high sensitivity and specificity. The detection of Dsg antibody level can be used for diagnosis and guidance of state and treatment of the disease.

Objective: To study the changes and significance of CD4 and CD8 expression in skin lesions of patients with erythema nodosum. Methods: MaxVision ^TM immunohistochemical stain was used to detect the expression of CD4 and CD8 in skin lesions of 49 cases of erythema nodosum patients (disease group) and 10 cases of normal skin (control group). Results: The CD4 positive rate of disease group was 100.00%, which was obviously higher than control group (P<0.01). There was no significant difference of positive rate of CD8 expression between disease group and control group (P>0.05). Conclusions: There is an imbalance of T cells subgroups in skin lesions of erythema nodosum patients, which suggests that the cellular immune response mediated by CD4 ⁺ T cells plays an important role in the pathogenesis of erythema nodosum.

Objective: To evaluate the intervention effects of parenteral nutrition with different nitrogen-content onnutriture and immune indice of patients with gastrointestinal tumors after surgery. Methods: 50 patients with gastrointestinal tumors after surgery were randomly divided into experimental group and control group with 25 patients in each group. All the patients were given total parenteral nutrition through central vein on the first day after operation, and the non-protein calorie were all 83.7-104.6kJ/(kg•d). Meanwhile, the nitrogen-content of experimental group was 0.20g(0.15-0.22g)/(kg•d), while the control group was 0.10g(0.09-0.11 g)/(kg•d). The hemoglobin, albumin, CD ₃ ⁺, CD ₄ ⁺ and CD ₄ ⁺/CD ₈ ⁺ of patients in 2 groups on the 14 ^th day after operation were compared. Results: 14 ^th day after operation, the hemoglobin, albumin, CD ₃ ⁺, CD ₄ ⁺ and CD ₄ ⁺/CD ₈ ⁺ of patients in experimental group were all obviously higher than control group (P<0.05). Conclusions: On the basis of giving the same heat quantity, total parenteral nutrition provided by normal nitrogen-content [0.20g/(kg•d)] can improve the nutritional and immune status of patients after gastrointestinal tumor surgery.