Objective To investigate the effect of skeletal muscle contusion on iron metabolism and its correlation with ferroptosis in rats. Methods Male SD rats were randomly divided into control group and blunt contusion model group. The model group was selected at 1d, 3d, 5d, 7d and 14d after injury, respectively. HE staining was used to observe the pathological changes of the injured tissue, and Prussian blue staining was used to observe the iron ion deposition in the injured area. ELISA method was used to detect the changes of serum ferritin, muscle tissue reactive oxygen species, α-ketoglutarate and heat shock protein 1. Results HE staining showed the rupture of muscle membrane, nucleolysis and inflammatory cell infiltration in the contusion area, which began to decrease after 5 days. The number of new skeletal muscle cells in the contusion area increased further on 14 days, and the number of intercellular collagen fibers increased. Prussian blue staining showed that iron deposition increased gradually after contusion, the highest in fifth day group, and then decreased. ELISA results showed that serum ferritin, ROS and α-KG in the model group increased gradually after injury, and the difference was statistically significant compared with the control group (P<0.01). HSPB1 first increased and then decreased after injury, and the difference was statistically significant compared with the control group (P<0.01). Conclusion Abnormal iron metabolism is closely related to ferroptosisafter skeletal muscle contusion, and cell ferroptosis also occurs after skeletal muscle acute injury.