Objective To investigate the relationship between the neutrophil to lymphocyte ratio (NLR), the platelet to lymphocyte ratio (PLR) and the degree of carotid stenosis. Methods The 236 patients who received angiography were selected as study objects. According to the angiography results, the patients were divided into the pathological group and the non-pathological group, and the NLR and PLR of the two groups were compared. The relationship between NLR and PLR and the degree of carotid artery stenosis was analyzed according to the degree of carotid artery stenosis of the patients, and ROC curve was plotted. Results Compared with patients without carotid artery stenosis, PLR and NLR increased in patients with carotid artery stenosis (P<0.05), with statistically significant differences. NLR and PLR were positively correlated with the degree of carotid artery stenosis in the patients(P<0.05), indicating a statistically significant difference. With severe and occlusion of carotid stenosis of PLR and NLR ROC curve analysis and calculate the area under the curve (motorcycle under the curve, AUC). PLR and NLR have predictive value (P<0.05), the AUC of PLR is 0.613, the best capture a value of 128.41, sensitivity of 63%, specificity of 58.7%, the AUC of NLR is 0.616, the best capture a value of 2.76, sensitivity of 52.1%, specificity of 74.6%. Conclusion NLR and PLR are valuable in predicting the degree of carotid stenosis.

Objective: To investigate the protective effects of pcDNA3.1(+)-SjP14 Nano microsphere nucleic acid vaccine on liver injury induced by Schistosoma japonicum in mice. Methods: 30 BALB/c mice were randomly divided into normal saline (NS) group, pcDNA3.1(+)-SjP14 group and Nano microsphere nucleic acid vaccine group, with 10 mice in each group. The mice in each group were respectively injected NS,pcDNA3.1(+)-SjP14 recombinant plasmid and Nano microsphere nucleic acid vaccine through quadriceps femoris (100μg /time,1 time/2 weeks,3 times).2 weeks after the last injection,all the mice were infected by cercaria of S. japonicum,the mice were killed 6 weeks after infection. HE staining was used to observe the pathological changes of liver; as well as the number of eggs, the average diameter, area and volume of single egg granuloma were calculated. Results: General observation and HE staining all showed that the liver pathological changes of mice in NS group were the most serious, while the Nano microsphere nucleic acid vaccine group were the slightest. The number of eggs,the average diameter, area and volume of single egg granuloma of mice in Nano microsphere nucleic acid vaccine group were obviously lower than NS group and pcDNA3.1(+)-SjP14 group (P<0.05). Conclusions: The pcDNA3.1(+)-SjP14 Nano microsphere nucleic acid vaccine has certain protective effects on liver injury induced by S. japonicum in mice.