Objective To investigate the correlation between CD40 ligand (CD40L), soluble FMS like tyrosine kinase receptor-1 (sFlt-1), salivary liquefied sugar chain antigen-6 (KL-6) and the prognosis of patients with severe pneumonia (SP). Methods Ninety-eight SP patients admitted to Zhengzhou Third People's Hospital from January 2020 to December 2022 were selected as the study objects. According to the disease outcomes 28 days after admission, the patients were divided into a good outcome group (69 cases) and a poor outcome group (29 cases). Serum levels of CD40L, sFLT-1 and KL-6 were compared between the two groups, and the pulmonary function indicators [forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), and peak expiratory flow (PEF)] of the two groups were compared, and the correlation between the levels of serum indicators and pulmonary function indicators and disease outcomes was analyzed, and the risk of adverse outcomes of patients with different levels of indicators was analyzed. Results After 7 and 14 days of treatment, the levels of CD40L, sFLT-1 and KL-6 in the poor group were higher than those in the good group, and the levels of FVC, FEV1 and PEF were lower than those in the good group (P<0.05). Serum CD40L, sFLT-1 and KL-6 levels were negatively correlated with disease outcome, FVC, FEV1 and PEF after 7 and 14 days of treatment (P<0.05). After 14 days of treatment, the risk of adverse outcomes in patients with high levels of serum CD40L, sFLT-1 and KL-6 was 3.255, 3.911 and 2.322 times that of low levels, respectively (P<0.05). Conclusion Serum CD40L, sFLT-1 and KL-6 levels are closely related to the disease outcome of SP patients, and can be used as effective indicators to predict the outcome of SP patients.

Objective To detect the expression of ZMYND8 in gastric cancer tissuesand their adjacent tissues, clarify the relationship between the expression level of ZMYND8 and the clinicopathological parameters of gastric cancer patients, and analyze the relationship between the expression of ZMYND8 and the 5-year survival rate of gastric cancer patients. Methods The expression of ZMYND8 mRNA in 30 cases of gastric cancer fresh tissues and the adjacent tissues were detected by RT-qPCR. Immunohistochemical was used to detect the expression of ZMYND8 protein in 60 cases of tumor and paraffinic samples. The correlation between the ZMYND8 expression and the survival rate of patients was analyzed by K-M survival curve. Results The expression of ZMYND8 mRNA in gastric cancer was significantly lower than that in the adjacent tissues (P<0.01). The positive rate of ZYND8 protein expression in gastric cancer was 21.6%, which was significantly lower than that in the adjacent tissues (85.0%, P<0.05). ZMYND8 expression was closely related to the depth of tumor cell invasion, lymph node metastasis and TNM stage (P<0.05). The results of K-M survival analysis showed that the 5-year survival rate of patients with positive expression of ZMYND8 was significantly higher than that of patients with low expression (P<0.01). Conclusion The expression rate of ZMYND8 in gastric cancer is low and closely related to tumor invasion, lymph node metastasis, TNM stage and 5-year survival rate of patients, which suggest that detection of the expression of ZMYND8 is of great significance in predicting the prognosis of gastric cancer patients.