Objective To investigate the prevalence and species of tick-borne Rickettsiales in rural areas of Chengde City, and to provide a theoretical basis for the prevention and control of diseases caused by tick-borne Rickettsiales. Methods Total DNA was extracted from ticks collected from Sangou Town and Liugou Town of Chengde City, and the 16S rRNA gene was amplified by nested PCR to identify Rickettsiae bacteria. The heat shock protein(groEL) gene of Anaplasma and outer membrane protein A (ompA) gene of Rickettsia were amplified and sequenced for homology and phylogenetic analysis to identify the pathogens of tick-borne zoonotic Anaplasma and Rickettsia. Results A total of 336 ticks were collected from Sangou Town and Liugou Town, and all of them were identified as Haemaphysalis longicornis. The positive rate of Anaplasma was 25.3% (85/336) in ticks and 17.3% (58/336) in Rickettsia. The positive rate of Anaplasma in ticks was significantly higher than that in Rickettsia (P=0.01). The positive rates of Anaplasma capra and Anaplasma ovis were 8.3% (28/336) and 17.0% (57/336), respectively. The positive rate of Rickettsia sibirica and Rickettsia raoultii was 3.6% (12/58) and 6.3% (21/58), respectively. The positive rate of Candidatus Rickettsia jingxinensis was 7.4% (25/58). Conclusion Two zoonotic Anaplasma and three Rickettsia species were identified in H. longicornis in Chengde, indicating that Rickettsiae bacteria prevalent in rural areas around Chengde present high genetic diversity. And it is necessary to strengthen the monitoring and prevention and control of ticks and tick-borne pathogens in Chengde.