Objective To investigate the mechanistic role of LncRNA MITA1 in the progression of hepatocellular carcinoma (HCC). Methods qRT-PCR was employed to assess the expression levels of MITA1 mRNA in HCC tissues and cells. The pcDNA3.1 recombinant plasmid vector was utilized to overexpress MITA1. After treatment, SMMC7721 cells were categorized into three groups, the normal group, the OE-MITA1-NC (negative control) group, and the OE-MITA1 (overexpression) group. Cell proliferation was evaluated using a colony formation assay, while cell invasion was assessed via the Transwell assay. Cell viability was determined using the Cell Counting Kit-8 (CCK-8) assay. Protein expression levels of Vimentin, MMP-2, and N-Cadherin were measured by western blotting. Additionally, the dual-luciferase reporter assay was conducted to verify the targeting relationship between MITA1 and miR-30b-3p. Results Compared with adjacent normal liver tissues, HCC tissues and cells exhibited significantly higher expression levels of MITA1 mRNA and lower expression levels of miR-30b-3p mRNA (P<0.05). Under MITA1 overexpression conditions, the OE-MITA1 group demonstrated elevated MITA1 mRNA expression levels (P<0.05). Compared with the normal and OE-MITA1-NC groups, the OE-MITA1 group exhibited increased cell proliferation, invasion capacity, and enhanced cell viability (P<0.05). Furthermore, protein expression levels of Vimentin, MMP-2, and N-Cadherin were significantly higher in the OE-MITA1 group compared with the other two groups (P<0.05). The dual-luciferase reporter assay confirmed a targeting relationship between MITA1 and miR-30b-3p, with MITA1 expression negatively regulating miR-30b-3p expression. Conclusion LncRNA MITA1 can promotes the proliferation, invasion and cell activity of liver cancer by binding miR-30b-3p, MITA1 may serve as a potential therapeutic target for the diagnosis and treatment of HCC.

Objective To explore the application effect of day surgery for breast benign tumor resection after medical insurance payment in Wuyi, Jiangmen. Methods From January 2019 to December 2019, 160 patients who underwent breast benign tumor resection in Jiangmen Central Hospital were selected as the research objects. Taking June 30, 2019 as the time node, patients were divided into observation group and control group, 80 cases in each group. The control group adopted the routine in-hospital operation process and discharged within 72 hours. The observation group adopted daytime operation process, outpatient preoperative examination, appointment for breast benign tumor resection and observation operation. The patients were discharged within 24 hours. The hospitalization time, hospitalization cost, operation time, number of tumor resection, postoperative complications and patient satisfaction were compared between the two groups. Results The length of stay in the observation group was significantly shortened, the cost of stay in the observation group was significantly reduced, and patients' satisfaction was higher, the difference was statistically significant (P<0.05). There were no significant differences between the two groups in terms of operation time, number of tumor resection and postoperative complications (P>0.05). Conclusion In Wuyi area of Jiangmen, the application of daytime operation process in breast benign tumor resection can effectively shorten the length of stay, improve the turnover rate of beds in the department, save the cost of medical insurance, and improve the satisfaction of patients, which has a good promotion value in clinical practice.