Objective To study the effect of TLE brain dysfunction on depression by analysing the brain electrical activity, and find out the functional specific brain regions related to TLE complicated with depression, so as to further assist clinical diagnosis and treatment. Methods According to Hamilton depression scale score, a total of 30 patients with temporal lobe epilepsy were collected and divided into 15 patients with temporal lobe epilepsy comorbidities depression group and 15 patients with non-depression group. All the 30 patients underwent VEEG examination, 3.0T MRI and RS-fMRI examination, and then the image data were preprocessed and statistically analyzed. Results There were no significant differences in age, sex between the two groups (P>0.05). The difference in duration of epilepsy was statistically significant(P<0.05). Dominant side discharge was more common in depression group. The independent t-test of two samples which based on three kinds of RS-fMRI analysis methods(amplitude of low frequency fluctuations, fractional alff and regional homogeneity) showed that stronger activate brain regions were included the cingulum, the precuneus, the parietal which belonged to default-mode network. Conclusion Depression patients with temporal lobe epilepsy comorbidities generally have a longer course of disease, and more dominant hemispheres are involved. The activation of the brain default network and the abnormal structure of brain regions are related to the generation of depression. The technique can be applied to guide diagnosis, treatment and surgical intervention.