Objective To detect the expression characteristics of lncRNA SNHG16 in hepatocellular carcinoma, and observe the proliferation regulatory effect by miR-141-3p/FOXJ3 on the hepatocarcinoma cells. Methods Potential binding sites were predicted between SNHG16 and miR-141-3p, miR-141-3p and FOXJ3 by bioinformatics analyses. Forty-nine cases of hepatocellular carcinoma were collected, the tumor tissue and the adjacent liver tissue were retained. The normal human hepatocyte HL-7702, hepatocarcinoma cells of Hep-3B and Huh7 were selected. The pc-DNA3.1-SNHG16 group, si-SNHG16 group, miR-141-3p inhibitor group, miR-141-3p mimic group, and si-FOXJ3 group were constructed The cell proliferation activity was detected through CCK-8 method. The expression of SNHG16 and miR-141-3p was detected by real-time fluorescence quantitative PCR(qRT-PCR), FOXJ3 and Ki67 were detected through immunohistochemistry method, FOXJ3 were detected by Western blot. Target relationship was verified between SNHG16 and miR-141-3p, miR-141-3p and FOXJ3 by double luciferase reporter gene experiment. Results Possible combination of base sequences were found between SNHG16 and miR-141-3p, miR-141-3p and FOXJ3 by bioinformatics analysis. The expression of SNHG16 and FOXJ3 were higher in human hepatoma cell line than in normal hepatocyte line. The expression of miR-141-3p was lower in human hepatoma cell line than that in normal hepatocyte line. Compared with the control group and the empty vector transfection group, the proliferation activity was increased at 48h in pc-DNA3.1-SNHG16 group, while it was decreased in si-SNHG16 group, which lasted to 96h. Target relationship was found between SNHG16 and miR-141-3p, miR-141-3p and FOXJ3 in double luciferase reporter gene test. The rescue experiment showed that the inhibition of miR-141-3p could partially reverse the inhibition effect of si-SNHG16 and si-FOXJ3 on cell proliferation. The expression of SNGHN16 and the positive rate of FOXJ3 in hepatocellular carcinoma were significantly higher than that in adjacent liver tissues, and the expression of miR-141-3p was significantly lower than that in adjacent liver tissues. SNHG16 was negatively correlated with miR-141-3p, miR-141-3p and FOXJ3, and positively correlated with FOXJ3 and Ki67 in hepatocellular carcinoma. Conclusion Expression of SNHG16 is increased in hepatocellular carcinoma, which promotes the proliferation through miR-141-3p/FOXJ3 pathway.

Objective To systematically evaluate the related factors affecting the prognosis of stroke patients with dysphagia, so as to provide evidence for the rehabilitation guidance of clinical prognosis of stroke patients with dysphagia. Methods The related studies on the prognostic factors of dysphagia in stroke were searched in the databases of CNKI, WanFang, Cochrane Library, PubMed and Web of Science. The search time was limited from January 1, 2010 to May 1, 2020. Meta analysis was performed by software RevMan5.3. Results A total of 11 articles were included. The results of Meta analysis showed that age≥70 years old (OR=0.16, 95%CI), cognitive impairment (OR=0.11, 95%CI), sensory aphasia (OR=0.18, 95%CI), dysphagia degree (OR=0.11, 95%CI), rehabilitation intervention time (OR=-6.10). 95%CI (-6.54), brainstem damage (OR=0.14, 95%CI), BI<60 (OR=0.42, 95%CI) and NIHSS score≥12 (OR=0.20, 95%CI) were the factors affecting the prognosis of dysphagia in stroke. Conclusion The prognostic effect of stroke patients with dysphagia is affected by many factors. Targeted preventive intervention measures should be given to the controllable factors in order to speed up the rehabilitation process, lighten the burden and promote the patients to return to society as soon as possible.

Objective To investigate the effect of core stability training in the treatment of lumbar degenerative diseases after percutaneous endoscopic discectomy. Methods A total of 60 patients with LDD, who were admitted to the spinal surgery department of hospital and received PELD treatment from October 2019 to December 2019, were selected as study subjects by the convenience sampling method. According to the methods of rehabilitation, 30 patients who received core stabilization training nursing after PELD surgery as the observation group and 30 patients who received routine nursing after PELD surgery as the control group. VAS and ODI scores of the two groups were compared before surgery, 1 week after surgery and 1 month after surgery, respectively. Results The VAS score and ODI score of the observation group were significantly lower than that of the control group (P<0.05). Conclusion The core stability training method can reduce the postoperative pain of LDD patients, improve the clinical efficacy, and is worthy to be clinically popularized.

Objective: To evaluate the effects of cervical single-door laminoplasty that preserving insertion of semi-spinus muscle on C ₂ vertebral body for treating cervical spondylotic melopathy. Methods: 60 patients with cervical spondylotic myelopathy were divided into group A and group B according to different surgical methods, 30 patients in each group. The patients in group A received C _3-7 cervical single-door laminoplasty, the patients in group B received C _4-7 cervical single-door laminoplasty. The JOA scores of patients before and at the last follow-up were analyzed, and the improvement rate of neurological function was calculated; The cervical curvature (Cobb angle) of the two groups before and at the last follow-up were compared; And the incidence rate of postoperative axial symptoms, C ₅ nerve root paralysis were recorded. Results: At the last follow-up, the cervical curvature of patients in 2 groups was obviously lost compared with that before operation (P<0.05); At the last follow-up, the Cobb angle of patients in group A was obviously lower than group B (P<0.05). There was no significant difference in JOA score before and after operation, neurological improvement rate and incidence rate of complications between group A and group B (P>0.05). The JOA score of patients in 2 groups after operation were all obviously higher than than before operation (P>0.05). Conclusions: Treating cervical spondylotic myelopathy with C _3-7 and C _4-7 cervical single door laminoplasty all can get significant recovery of neurological function. While the cervical single-door laminoplasty with preservation of hemi-spinus muscles insertion on C ₂ vertebral body not only can maintain the cervical curvature, but also can decrease the incidence rate of axial symptoms.

Objective: To study the spasticity of rat tail and the changes of dopamine receptor-1 (DRD1) expression in spinal cord after spinal cord injury (SCI). Methods: Adult male Wistar rats were randomly divided into sham operation group and SCI group. The 2 ^nd sacral spinal cord (S ₂) of rats in SCI group were transected totally; And then the tail spasticity score of rats were evaluated 2d, 7d, 14d, 30d and 60d after operation respectively. Immunofluorescence was used to detect the DRD1 expression in gray matter of sacral and coccygeal spinal cord 60d after operation. Results: The tail spasticity score of rats in SCI group 2d, 7d, 14d, 30d and 60d after operation were respectively 0, 1.10±0.28, 1.32±0.85, 2.50±0.53 and 4.15±0.36. DRD1 expression could be observed in dorsal horn (DH), intermediate zone (IMZ) and ventral horn (VH) in gray matter of sacral and coccygeal spinal cord of rats. The DRD1 expression in DH, IMZ and VH in gray matter of spinal cord of rats in SCI group were significantly lower than rats in sham operation group (P<0.05). Conclusions: Decreased expression of DRD1 in spinal cord after SCI may be associated with development of tail spasticity.

Objective: To investigate the protective effects of soybean isoflavone on hippocampal cholinergic neurons of chronic cerebral ischemia rats. Methods: 30 healthy, adult female SD rats were applied in this study and randomly divided into sham operation group (n=10), model group (n=10) and treatment group (n=10). The rats in model group and treatment group were established chronic cerebral ischemia model by permanently ligating the common carotid arteries on both sides of castrated rats. In sham operation group, the bilateral ovaries and common carotid arteries were only separated without ligature. After the chronic cerebral ischemia rats' model was established, the rats in treatment group were lavaged with soybean isoflavone (9mg/kg/d) for 21 days. SP immunohistochemical staining and MiVnt image analysis system were used to detect and analyze the choline acetyltransferase (ChAT) protein expression in CA1 region of hippocampus of rats in each group. Results: The ChAT protein expression of neurons in CA1 region of hippocampus of rats in sham operation group was obvioulsy higher than model group and treatment group (P˂0.05). The ChAT protein expression of neurons in CA1 region of hippocampus of rats in treatment group was obvioulsy higher than model group (P˂0.05). Conclusions: Soybean isoflavone has protective effects on cholinergic neurons in hippocampus of rats with chronic cerebral ischemia, the mechanisms may be related to soybean isoflavone can up regulate ChAT protein expression in hippocampus.

Objective: To investigate th regulatory effects of sericin on liver insulin receptor (IR) in type 2 diabetic rats. Methods: Thirty-six SPF male rats were randomly divided into three groups (normal group, model group and experimental group), with 12 rats in each group. The rat model of type 2 diabetes mellitus was induced by high-fat, high-sugar feeding and intraperitoneal injection of streptozotocin. After modeling, the rats were given sericin by gavaging for 35 days in the experimental group. The glucose oxidase method was used to detect the fasting blood glucose of rats in each group. Real Time Q-PCR was used to detect the expression of IR mRNA in liver. Results: Compared with the rats in model group, the blood glucose of rats in experimental group was significantly reduced, and the expression of liver IR of rats in experimental group increased significantly (P< 0.05). Conclusions: Sericin may enhance the transduction effects of insulin signal by up regulating the expression of IR in liver of diabetes mellitus rats, thus reducing the blood glucose.