Objective To find a convenient, fast, accurate, and cost-saving method for the puncture procedure of intracranial hematoma. Method One hundred patients with intracranial hematoma were selected for puncture treatment. According to the midline position of the CT film, mark the projection positions of the hematoma center on the horizontal plane, coronal plane, and sagittal plane of the body surface respectively. One of the marking points can be used as a puncture point, and the plane of the other two targets can be used as the maximum level and puncture angle of the hematoma, which is called the “one point two planes” method. Results After the puncture procedure, using the “one point two planes” method for positioning. The puncture points were in the hematoma center verified by CD film in the 100 patients. Conclusion The “one point two planes” method is convenient, fast, accurate, and cost-saving method for the puncture procedure of intracranial hematoma and is suitable for basic-level hospitals to treat patients with acute cerebral hemorrhage.

Objective To investigate the effects of early intervention with different doses of hesperetin on learning and memory ability, amyloid beta protein 1-42(Aβ42) and neprilysin (NEP) activity in APPswe/PS1dE9 double transgenic mice. Methods Three month old C57BL/6J wild-type mice were used as control group(0.5% CMC-Na), and three month old APPswe/PS1dE9 double transgenic mice were used as model group, hesperetin low, medium and high dose groups (0, 20, 40 and 80 mg/kg/d), respectively. They were gavaged once a day for 6 months. Morris water maze behavior test was used to observe the learning and memory ability of mice; HE staining was used to observe the morphology of hippocampal neurons; ELISA was used to detect the content of Aβ 42 in serum; Western blot was used to detect the expression of Aβ 42 and neprilysin (NEP) in brain tissue. Results Compared with the control group, the incubation period of mice in the model group was significantly prolonged(P<0.05), the number of crossing platforms was reduced(P<0.05), the neurons in the hippocampal CA1 area were significantly damaged, the serum Aβ42 content was significantly increased(P<0.05), the content of Aβ42 in the brain tissue was significantly increased(P<0.01), and there was no significant difference in the content of NEP. Compared with the model group, the incubation period of mice in the low, medium and high dose groups of hesperetin was significantly shortened(P<0.01). The number of times increased(P<0.01), the morphological structure of hippocampal CA1 neurons was significantly improved, the content of Aβ42 in serum was significantly reduced(P<0.01), the content of Aβ42 in brain tissue was significantly reduced(P<0.01). The NEP content in the medium and high dose group were significantly increased(P<0.01). Conclusion Early intervention of hesperetin could significantly improve the learning and memory ability of APPswe/PS1dE9 double transgenic mice and neuronal damage in hippocampus CA1 area. The mechanism might be related to the increase of NEP activity and the enhancement of Aβ42 metabolism.

Objective: To study the effects of picropodophyllin on proliferation of human colorectal cancer HCT-15 cells. Methods: Human colorectal cancer HCT-15 cells were cultured in vitro. The proliferative activity of HCT-15 cells after treating with different concentrations of picropodophyllin for 24h, 48h and 72h were respectively detected by CCK-8 assay, and the morphological changes of HCT-15 cells were observed under inverted microscope. Results: CCK8 results showed that picropodophyllin could significantly inhibit the proliferation of HCT-15 cells. In the range of 0.125μmol/L~2μmol/L, the inhibition rate of HCT- 15 cells gradually increased with the increasing of picropodophyllin concentrations and action time, in a dose-time dependent manner. Under inverted microscope, the HCT-15 cells appeared shrikage, refractive reduction and decreasing of living cells at different degree after picropodophyllin treatment. Conclusions: Picropodophyllin has significant inhibitory effects on human colorectal cancer HCT-15 cells.

Objective: To investigate the expression and significance of c-met, vascular endothelial growth factor C (VEGF-C) and lymphatic microvessel density (LMVD) in esophageal squamous carcinoma. Methods: Immunohistochemical staining was used to detect the expression of c-met and VEGF-C, and LMVD was calculated by D2-40 in 25 cases of normal esophageal mucosa and 60 cases of esophageal squamous carcinoma; and the relationships between c-met, VEGF-C, LMVD and clinicopathologic features of esophageal squamous carcinoma were also analyzed. Results: The expression of c-met, VEGF-C and LMVD in esophageal squamous carcinoma were significantly higher than normal esophageal mucosa (P<0.05). c-met and VEGF-C expression in esophageal squamous carcinoma were related to lymph node metastasis and TNM stage (P<0.05); while LMVD was related to infiltration depth, lymph node metastasis and TNM stage (P<0.05). Conclusions: c-met, VEGF-C and lymphoangiogenesis may play important role in development, invasion and metastasis of esophageal squamous carcinoma.

Objective: To explore the expression and clinical significance of insulin-like growth factor 1 (IGF-1) in human breast cancer. Methods: MaxVision immunohistochemical staining and RT-PCR were used to detect the IGF-1 protein and mRNA expression in 80 cases of breast cancer, 60 cases of breast fibroadenoma and 50 cases of normal breast tissue adjacent to carcinoma; And the relationships between IGF-1 expression and clinicopathologic features of breast cancer were also analyzed. Results: The IGF-1 protein and mRNA expression in breast cancer were obviously higher than breast fibroadenoma and normal breast tissue adjacent to carcinoma (P<0.05). The expression of IGF-1 in breast cancer was not related to age (P>0.05), but was related to tumor size, lymph node metastasis and histological differentiation (P<0.05). Conclusions: High expression of IGF-1 may play important role in development of breast cancer.