Objective To investigate the correlations between carotid intima media thickness (CIMT) in patients with type 2 diabetes mellitus and omentin-1, visfatin, glutamyltranspeptidase. Methods The 116 patients with type 2 diabetes mellitus were divided into thickening group (CIMT≥0.9mm) and non-thickening group (CIMT<0.9mm) according to the CIMT. The omentin-1, visfatin and glutamyltranspeptidase in serum of patients in 2 groups were measured. Results The CIMT and visfatin of patients in thickening group were significantly higher than those in non-thickening group (P<0.05), the omentin-1 were significantly lower (P<0.05). There was no significant difference in glutamyltranspeptidase between the two groups (P>0.05). Spearman correlation analysis showed that the CIMT of type 2 diabetic patients negatively correlated with omentin-1 (r =–0.726, P<0.05), but positively correlated with visfatin (r = 0.919, P<0.05). Multivariate logistic regression analysis showed that visfatin was the independent risk factor for CIMT thickening in type 2 diabetic patients. Conclusion Omentin-1 and visfatinare correlated with CIMTin patients with type 2 diabetesmellitus, which can be used as serological indexs for early diagnosis of carotid atherosclerosis.