Objective To investigate the correlation between the expression of MET mRNA in carotid artery plaques and the occurrence of ischemic cerebrovascular disease (ICVD), in order to provide reference for the prevention and treatment of ischemic cerebrovascular disease. Method 97 ICVD patients who underwent carotid endarterectomy in our hospital from January 2021 to March 2023 were included. They were divided into vulnerable, non vulnerable, and coexisting groups based on the nature of the detected plaques. The MET mRNA of the stripped plaques was detected using qRT PCR method, and the correlation between MET mRNA and plaque nature, ICVD subtypes, and ICVD severity was analyzed. Results The mRNA expression levels in the vulnerable group, non vulnerable group, and coexisting group were 0.763 ± 0.047, 0.591 ± 0.039, and 0.708 ± 0.043, respectively, with statistically significant differences (P<0.05). The mRNA expression levels among patients with transient cerebral ischemia, cerebral infarction, cerebral steal syndrome, and chronic cerebral ischemia were 0.711 ± 0.068, 0.765 ± 0.077, 0.655 ± 0.071, and 0.671 ± 0.083, respectively, with statistically significant differences (P<0.05). The mRNA expression levels among patients with mild, moderate, moderate, and severe NIHSS grading were 0.642 ± 0.072, 0.671 ± 0.074, 0.754 ± 0.039, and 0.789 ± 0.051 respectively, with statistically significant differences (P<0.05). The expression level of MET mRNA in carotid plaques was significantly correlated with plaque type, ICVD classification, NIHSS grading, carotid stenosis grading, fasting blood glucose, total cholesterol, triglycerides, high-density lipoprotein, and low-density lipoprotein, all P<0.05. Conclusion The expression of MET mRNA in carotid artery plaques is significantly correlated with the occurrence of ICVD, mainly by participating in the development of plaque instability leading to transient cerebral ischemia and stroke, and interacting with hyperglycemia and hyperlipidemia.

Objective: To investigate the clinical effects of iRoot BP on repairing perforation of pulp floor. Methods: Forty patients with perforation of pulp floor were selected in this study and randomly divided into observation group (n=20) and control group(n=20). The patients in observation group were repaired with iRoot BP, while the patients in control group were repaired with mineral trioxide (MTA). The success ratio of the reparation of patients in 2 groups 3 months, 6 months, 12 months after treatment were compared, as well as the incidence of complications and satisfaction rate of repair treatment of 2 groups were recorded and compared. Results: The success ratio of the reparation of patients in observation group 3 months and 6months were significantly higher than control group (P<0.05); There was no significant difference about success ratio of the reparation 12 months after treatment of 2 groups (P>0.05). None of the patients in observation group suffered occlusal pain and loose teeth, and the complication rate of observation group was 0.00%, which was significantly lower than control group (20.00%, P<0.05). The satisfaction rate of repair treatment of observation group was 95.00%, which was significantly higher than control group (65.00%, P<0.05). Conclusions: The treatment effects of iRoot BP for treating perforation of pulp floor are better than MTA, the safety and satisfaction rate of repair treatment are all higher, so it is worthy of clinical application.

Objective: To explore the best cut-off point of orthostatic plasma aldosterone/plasma renin concentration (ADRR) for screening primary aldosteronism (PA). Methods: 80 cases of patients with unknown causes of hypertension were divided into PA group (combined with PA, n=20) and primary hypertension group (hypertension group, not combined with PA, n=60). The body height, body weight, blood pressure, blood potassium, orthostatic plasma aldosterone concentration (PAC), plasma renin activity (PRA) and plasma direct renin concentration (PDC) were measured respectively of all the patients; And the PAC/PRA (ARR) and ADRR were calculated. The ROC curve was drawn to determine the best cut-off point of orthostatic ADRR screening for PA. Results: The serum potassium level, PDC and PRA of patients in PA group were significantly lower than hypertension group, while the PAC, ADRR and ARR were significantly higher than hypertension group (P<0.05). The area under ROC curve of ARR was 0.698, and the diagnostic cut-off for screening PA was 63.77 [(pg/ml)/(μg/L/h)]. The area under ROC curve of ADRR was 0.882, and the diagnostic cut-off for screening PA was 2.55 (pg/ml)/(pg/ml). Conclusions: The diagnostic value of ADRR screening for PA is higher than that of ARR. It is suggested that ADRR can be used in clinical screening for PA.

Objective: To analyze the relationships between apolipoprotein B/A1, glycosylated hemoglobin (HbA1c) level and venous thrombolytic outcome in acute cerebral infarction patients. Methods: 76 acute cerebral infarction patients were all treated with recombinant tissue plasminogen activator (rt-PA) venous thrombolysis, and the patients were divided into ineffective group (n=27) and effective group (n=49) according to improvement of NIHSS score before and after thrombolysis. The blood glucose, blood lipid, HbA1c and other indicators of all the patients were detected. Results: The HbA1c, ApoB and ApoB/A1 of patients in effective group were significantly lower than ineffective group, the ApoA1 was significantly higher (P<0.05). Logistic regression analysis showed that age, HbA1c and ApoB/A1 were independent risk factors for thrombolytic outcome (P<0.05). Conclusions: ApoB/A1 and HbA1c may has certain predicting value for thrombolytic outcome in acute cerebral infarction patients at early stage.

ObjectiveTo investigate the effects of type H hypertension on prognosis of intravenous thrombolysis in patients with acute cerebral infarction.Methods182 cases of acute cerebral infarction patients receiving intravenous thrombolysis with at-PA were divided into type H hypertension+cerebral infarction group (group A), hypertension+cerebral infarction group (group B), hyperhomocysteinemia+cerebral infarction group (group C) and cerebral infarction group without HHcy and hypertension (group D). The blood pressure, serum Hcy level and prognosis of patients in 4 groups were compared; And the influencing factors of poor prognosis after thrombolytic therapy were also analyzed.ResultsThe systolic pressure, serum Hcy level of patients in group A were obviously higher than group B, C, D (P<0.05). Compared with other 3 groups, the incidence of poor prognosis of group A was the highest (P<0.05). Logistic regression analysis showed that age, Hcy and systolic pressure are independent risk factors of recent poor prognosis of intravenous thrombolysis after acute cerebral infarction.ConclusionsType H hypertension is an important risk factor for the prognosis of intravenous thrombolysis in patients with acute cerebral infarction.

Objective: To investigate the curative effects for treating condyloma acuminatum of vulva with LEEP knife combined with imiquimod cream. Methods: 138 cases condyloma acuminatum of vulva patients were randomly divided into observation group and control group with 69 cases in each group. The patients in control group were treated with LEEP knife, the patients in observation group were treated with LEEP knife combined with imiquimod cream. The total effective rate 4-week and 8-week after treatment, as well as recurrence rate and adverse effects rate of 2 groups were compared. Results: The total effective rate 8-week after treatment of patients in 2 group was all significantly higher than 4-week after treatment (P<0.05); while the total effective rate 4-week and 8-week after treatment of patients in observation group were all significantly higher than control group (P<0.05). The recurrence rate and adverse effects rate of patients in observation group were respectively 8.7% (6/69) and 60.9% (42/69), which were obviously lower than that of control group (P<0.05). Conclusions: Treating condyloma acuminatum of vulva with LEEP knife combined with imiquimod cream has the advantages of good clinical efficacy, low recurrence rate and less adverse effects, so it is worthy of application in clinic.