Objective: To investigate the rationality of bilateral central lymph node dissection for unilateral differentiated thyroid cancer. Methods: 80 cases of unilateral differentiated thyroid cancer patients were all treated with radical thyroidectomy combined with bilateral central lymph node dissection, and the central lymph node metastasis and postoperative complications of patients were analyzed. Results: Among 80 patients, ipsilateral central lymph node metastasis in 32 patients (32/80, 40.0%), contralateral central lymph node metastasis in 8 cases (8/80, 10.0%), simultaneous metastasis of bilateral central lymph nodes in 16 cases (16/80, 20.0%). The total contralateral lymph node metastasis rate was 30.0% (24/80), the total lymph node metastasis rate was 70.0% (56/80). 9 (9/80, 11.3%) patients suffered temporary hypocalcemia, 8 (8/80, 10.0%) patients suffered temporary recurrent laryngeal nerve injury after operation. All the 80 patients were followed up for 3 years without recurrence or metastasis. Conclusions: Ipsilateral central lymph node is the most easily metastatic site of unilateral differentiated thyroid cancer, but contralateral central lymph node also has possibility of metastasis and skip metastasis. Bilateral central lymph node dissection for unilateral differentiated thyroid cancer can remove potential metastatic lymph nodes effectively and determine metastasis of the tumour, which is benefical for reducing recurrence after operation and guiding postoperative staging.

Objective: To investigate the effects of sericin on phosphatidylinositol-3-kinase (PI3K) mRNA expression in pancreas of type 2 diabetic rats. Methods: Male SD rats were randomly divided into three groups: normal control group, diabetic model group and sericin treatment group. The rat model of type 2 diabetes mellitus was established by feeding high-fat and high-sugar diet and intraperitoneal injection of streptozotocin (35mg/kg, twice). Then the rats in the sericin treatment group were given sericin (2.4g/kg/d) by gavage. Glucose oxidase method was used to detect the blood glucose of rats, and Real Time Q-PCR were used to detect the expression of PI3K mRNA in pancreas of rats in each group. Results: Compared with the normal control group, the blood glucose of diabetic rats increased significantly and the expression of PI3K mRNA in pancreas decreased significantly (P<0.05); Compared with diabetic rats, the blood glucose of sericin treated rats decreased significantly, and the expression of PI3K mRNA in pancreas increased significantly (P<0.05). Conclusions: Sericin may regulate insulin PI3K-Akt signaling pathway by increasing the expression of PI3K in pancreas, thus reducing blood glucose .

Objective: To investigate th regulatory effects of sericin on liver insulin receptor (IR) in type 2 diabetic rats. Methods: Thirty-six SPF male rats were randomly divided into three groups (normal group, model group and experimental group), with 12 rats in each group. The rat model of type 2 diabetes mellitus was induced by high-fat, high-sugar feeding and intraperitoneal injection of streptozotocin. After modeling, the rats were given sericin by gavaging for 35 days in the experimental group. The glucose oxidase method was used to detect the fasting blood glucose of rats in each group. Real Time Q-PCR was used to detect the expression of IR mRNA in liver. Results: Compared with the rats in model group, the blood glucose of rats in experimental group was significantly reduced, and the expression of liver IR of rats in experimental group increased significantly (P< 0.05). Conclusions: Sericin may enhance the transduction effects of insulin signal by up regulating the expression of IR in liver of diabetes mellitus rats, thus reducing the blood glucose.

ObjectiveTo analyze the changes of insulin receptor substrate-1 (IRS-1) mRNA expression in pancreas of type 2 diabetic rats and the regulatory effects of sericin.MethodsMale SD rats were randomly divided into normal control group, diabetic model group and sericin medication group. The type 2 diabetic rats' model was established by high fat and high sugar feed combined streptozotocin injection. The rats in sericin medication group were lavaged with sericin (2.4g/kg) after the diabetic model was successfully established. Glucose oxidase method was used to detect the blood glucose of rats, Real Time Q-PCR to detect the IRS-1 mRNA expression in pancreas.ResultsCompared with rats in normal control group, the blood glucose of rats in diabetic model group increased obviously, the IRS-1 mRNA expression in pancreas decreased obviously (P<0.05). Compared with rats in diabetic model group, the blood glucose of rats in sericin medication group decreased significantly, the IRS-1 mRNA expression in pancreas increased significantly (P<0.05).ConclusionsSericin may reduce blood glucose by up regulating the expression of IRS-1 in pancreas.

Objective: To investigate the effects of sericin on expression of Bcl-2 protein in rats’ INS-1 cells. Methods: INS-1 cells were divided into normal control group, streptozotocin (STZ)-injuried group and sericin-treatment group. The INS-1 cells in normal control group were cultured with complete medium without any medicine; the INS-1 cells in STZ-injuried group were cultured with complete medium containing 10mmol/L STZ; the INS-1 cells in sericin-treatment group were cultured with complete medium containing 10mmol/L STZ and 300μg/ml sericin. Western Blotting was used to detect the expression of Bcl-2 protein in INS-1 cells of each group. Results: Compared with normal control group, the Bcl-2 protein expression in INS-1cells of STZ-injuried group decreased obviously (P<; 0.05). Compared with STZ-injuried group, the Bcl-2 protein expression in INS-1 cells of sericin-treatment group increased obviously (P<; 0.05). Conclusions: Sericin may inhibit beta cell apoptosis by up regulating the expression of Bcl-2 protein.