ISSN 1004-6879
CN 13-1154/R
Journal of Chengde Medical University
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Regulatory Effects of Sericin on Glycolysis of Damaged INS-1 Cells through PI3K/Akt Signal Pathway
LI Yu-xin, HAN Si-yu, YI Meng-ya, LI Jing-yao, CHEN Zhi-hong
Abstract
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29
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Objective
To observe the regulatory effects of sericin on PI3K/Akt signal pathway and glycolysis of INS-1 cells injured by streptozotocin (STZ).
Methods
INS-1 cells cultured in vitro were randomly divided into five groups. Normal control group, model group, sericin group, Akt1 inhibitor group and Akt1 agonist group. Western blotting and real-time fluorescence quantitative PCR were used to detect the expression of phosphatidylinositol-3-kinase (PI3K), protein kinase B (Akt), phosphofructosekinase-1 (PFK1), 6-phosphofructose-2,6-diphosphatase (PFKFB2) protein and mRNA.
Results
Compared with the normal control group, the protein expression of PI3K, p-Akt, PFK1, PFKFB2 of INS-1 cells in the model group decreased significantly (P<0.05). The protein expression of PI3K, p-Akt, PFK1, PFKFB2 of INS-1 cells in the sericin group were significantly higher than that in the model group (P<0.05). The protein expression of p-Akt, PFK1, PFKFB2 of INS-1 cells in the Akt1 inhibitor group were significantly lower than that in the sericin group (P<0.05). Compared with sericin group, the protein expression of p-Akt, PFK1 and PFKFB2 showed an upward trend in the Akt1 agonist group. The change trend of PI3K, Akt, PFK1, PFKFB2 mRNA expression in INS-1 cells of each group were consistent with that of protein.
Conclusion
The protective mechanism of sericin on STZ-induced injury of INS-1 cells may be that targeted Akt1 affects PI3K/Akt signal pathway and enhances glycolysis.
2024, 41 (3): 181-184.
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Sericin Targeting Akt1 Regulates PI3K/Akt Signaling Pathway to Promote Proliferation of INS-1 Cells Damaged by STZ
HAN Si-yu, LI Yu-xin, YI Meng-ya, LI Jing-yao, CHEN Zhi-hong
Abstract
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33
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(7543KB)(
6
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Objective
To investigate whether sericin promotes proliferation of streptozotocin (STZ) damaged insulinoma cells (INS-1 cells) through targeting Akt1 regulates PI3K/Akt signaling pathway.
Methods
INS-1 cells were randomly divided into four groups. In control group, INS-1 cells were cultured under conventional conditions without other treatments. In model group, INS-1 cells were cultured with 10 mmol/L STZ. In sericin group, INS-1 cells were cultured with 10 mmol/L STZ and 600 μg/mL sericin. In inhibitor group, INS-1 cells were cultured with 10 mmol/L STZ, 600 μg/mL sericin and 0.3 mmol/L Akt1 inhibitor A-674563. The cells in four groups were cultured with corresponding drugs respectively for 24h. The survival rate of INS-1 cells in each group was detected by CCK-8 method. Western blot was used to detect the expression of PI3K/Akt signaling pathway related proteins PI3K and p-Akt, and proliferation related proteins PCNA and Ki67.
Results
The survival rate, and the protein expressions of PI3K, p-Akt1, PCNA, and Ki67 of INS-1 cells in model group significantly decreased compared with control group (P<0.05). The survival rate, and the protein expressions of PI3K, p-Akt1, PCNA, and Ki67 of INS-1 cells in sericin group significantly increased compared with model group (P<0.05). The survival rate, and the protein expressions of PI3K, p-Akt1, PCNA, and Ki67 of INS-1 cells in inhibitor group significantly decreased compared with sericin group (P<0.05).
Conclusion
Sericin can protect proliferation of INS-1 cells damaged by STZ, and the protective mechanisms may related to target Akt1 regulates PI3K/Akt signaling pathway.
2024, 41 (2): 96-100.
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Protective Effects of Sericin on STZ-induced Injury INS-1 Cells
LI Jin-yao, HAN Si-yu, LI Yu-xin, YI Meng-ya, CHEN Zhi-hong
Abstract
(
106
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(6358KB)(
33
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Objective
To explore the protective effects of sericin on streptozotocin(STZ)-induced injury rats’ INS-1 cells.
Methods
INS-1 cells were cultured in vitro and randomly divided into five groups. Normal control group (culture under conventional conditions), model group (10mmol/L STZ), low sericin group (STZ+150μg/mL sericin), medium sericin group (STZ+300μg/mL sericin), high sericin group (STZ+600μg/mL sericin). The cells in five groups were cultured respectively with corresponding drugs for 24h. The protein and mRNA expressions of BCL-2 and P53 were detected by Western blotting and real-time PCR. Cell apoptosis was detected by flow cytometry.
Results
Compared with the normal control group, the expression of BCL-2 protein and mRNA of INS-1 cells in the model group were remarkably decreased, while the expression of P53 protein and mRNA were remarkably increased (P<0.05), as well as the early apoptosis rate was remarkably increased (P<0.05). Compared with the model group, the expression of BCL-2 protein and mRNA in 3 sericin groups were remarkably increased, while the expression of P53 protein and mRNA were remarkably decreased (P<0.05), and the early apoptosis rate was remarkably decreased (P<0.05). Moreover, there were statistically remarkable differences among the 3 sericin groups (P<0.05).
Conclusion
The protective effects of sericin on STZ-induced injury INS-1 cells is related to the regulation of apoptotic proteins and inhibition of INS-1 cell apoptosis.
2023, 40 (2): 91-95.
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Sericin Protects INS-1 Cells from STZ Injury by Affecting Autophagy and Oxidative Stress
HU Wan-xiang, LI Jin-yao, LI Yu-xin, HAN Si-yu, CHENG Lu-yang, CHEN Zhi-hong
Abstract
(
114
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(2112KB)(
37
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Objective
To investigate whether sericin can protect streptozotocin (STZ) induced INS-1 cells injury by affecting autophagy and oxidative stress.
Methods
INS-1 cells were randomly divided into group A, B, C, D and E. In group A (normal group), INS-1 cells were cultured under conventional conditions for 24h without other treatments. In group B(STZ induced injury group), INS-1 cells were cultured with 10 mmol/L STZ for 24h. In group C, D and E, INS-1 cells were respectivly cultured with 10mmol/L STZ and different dose sericin (150μg/mL, 300μg/mL, 600μg/mL) for 24h. Western blot and real-time fluorescence quantitative PCR were used to detect the expressions of autophagy related gene protein and mRNA, and the reactive oxygen species(ROS) content in INS-1 cellswas detected by DCFH-DA ROS fluorescence probe.
Results
The expressions of LC3B-II/I, SIRT1, ATG5, BECLIN1, PINK1 proteins and mRNA in group C, D and E were all significantly higher than those in group B (P<0.05). The expression levels of each index in the three groups were group E>group D>group C, and the differences were statistically significant (P<0.05). The ROS content of INS-1 cells in group C, D and E were all obviously lower than that in group B (P<0.05). The ROS content of the three groups was in the order of group E<group D<group C, and the difference was statistically significant(P<0.05).
Conclusions
ericin can protect STZ induced INS-1 cells injury, and the protective mechanism is related to sericin's ability to reduce oxidative stress and enhance autophagy.
2022, 39 (3): 186-192.