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PREVENTIVE EFFECTS OF POLYGALA ON DORSAL ROOT GANGLIA NEURONS INJURY IN RATS WITH DIABETIC PERIPHERAL NEUROPATHY
LI Ying, MA Hong-wei, FU Xiu-mei, et al
Objective: To investigate the preventive effects of Polygala on sciatic nerve-related neurons injury of diabetic peripheral neuropathy (DPN) rats.
Methods: The Wistar rats were randomly divided into 3 groups: blank control group, DPN model group and Polygala prevention group (n=12). The rats in DPN model group and Polygala prevention group were made streptozotocin induced diabetes model. Then the rats in Polygala prevention group were lavaged with Polygala (2.7g/kg/d) for 6 weeks. The DPN rats' model was established with SNCV˂30m/s as the standard. The immunohistochemical staining was used to detect the number of Bcl-2 and Bax positive cells in sciatic nerve-related dorsal root ganglia; Western Blot to detect Bcl-2 and Bax expression in dorsal root ganglia and TUNEL to detect apoptotic index of neurons in dorsal root ganglia.
Results: Compared with blank control group, the Bcl-2 immuno-positive cells and Bcl-2 expression in dorsal root ganglia of DPN rats decreased obviously (P˂0.01); while the Bax immuno-positive cells, Bax expression and apoptotic index in dorsal root ganglia increased obviously (P˂0.01). Compared with DPN model group, the Bcl-2 immuno-positive cells and Bcl-2 expression in dorsal root ganglia of rats in Polygala prevention group increased obviously (P˂0.01); while the Bax immuno-positive cells, Bax expression and apoptotic index in dorsal root ganglia of rats in Polygala prevention group decreased obviously (P˂0.01).
Conclusions: Polygala can inhibit neurons apoptosis in dorsal root ganglia of DPN rats by upregulating Bcl-2 expression and downregulating Bax expression, so has preventive effects on sciatic nerve-related neurons injury.
2019, 36 (4):
274-277.
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