Objective To screen key genes and pathways associated with tumorigenesis and progression of triple-negative breast cancer (TNBC) by using bioinformatics analysis. Methods Two gene expression profilings containing TNBC (GSE76124) and normal mammary (GSE112825) tissue samples were obtained from Gene Expression Omnibus (GEO). R software was used to identify differentially expressed genes. Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were further performed by DAVID. STRING and Cytoscape were used to construct protein-protein interaction(PPI) network. Furthermore, hub genes, core modules and seed genes were screened out. Results A total of 1,091 differentially expressed genes were screened out. The results of GO function analysis and KEGG pathway maps showed that these genes were associated with aromatic compound biosynthetic process, heterocycle biosynthetic process, and mainly enriched on the pathways in cancer and PI3K-Akt signaling pathway.We identified 10 hub genes, 2 core modules and 19 seed genes in PPI network. Conclusion A total of 10 hub genes and 19 seed genes were screened, which may play potential roles in the tumorigenesis and progression of TNBC. This study provids a bioinformatic analysis reference for further research.

Objective: To compare the effects of fragment cryopreservation of mouse ovarian tissue and whole cryopreservation of mouse ovarian tissue on vitrification. Methods: Mouse ovarian tissues were randomly divided into fragment cryopreservation group, whole cryopreservation group and fresh control group. The ovarian tissue of mice in fresh control group were immediately fixed with 4% paraformaldehyde after taking out; The ovarian tissue of mice in fragment cryopreservation group were cryopreserved after cut into 1×1×1 mm ³ cubes; The ovarian tissue of mice in whole cryopreservation group were cryopreserved directly with no cutting. The rate of normal morphological follicles were calculated and the diameter of all kinds of follicles were measured after the ovarian tissues embedding and HE staining as normal. Results: The normal morphological follicles rate of ovarian tissues in fragment cryopreservation group and whole cryopreservation group were significantly lower than fresh control group (P<0.01); There had no statistical significance about normal morphological follicles rate between fragment cryopreservation group and whole cryopreservation group (P>0.05). The diameter of Primordial follicle, primary follicle, secondary follicle, sinus follicle of ovarian tissues in fragment cryopreservation group and whole cryopreservation group were significantly higher than fresh control group (P<0.05); There had no statistical significance about diameter of all kinds of follicles between fragment cryopreservation group and whole cryopreservation group (P>0.05). Conclusions: Whole cryopreservation of mouse ovarian tissue does not affect the effects of vitrification.