Objective:To investigate the expression of epithelial-mesenchymal transition (EMT) markers in colorectal cancer and the relationships with malignancy of colorectal cancer.Methods:SP immunohistochemical method was used to detect the expression of EMT markers, E-cadherin, β-catenin and Vimentin in 131 cases of colorectal cancer tissues and para-carcinoma tissues. Results:The positive expression rate of E-cadherin and β-catenin in colorectal cancer tissues were significantly lower than para-carcinoma tissues, the positive expression rate of Vimentin was significantly higher (P<0.05). The positive expression rate of E-cadherin and β-catenin in low differentiated colorectal cancer were significantly lower than high-to-middle differentiation, The positive expression rate of Vimentin was significantly higher (P<0.05). Conclusions:EMT is related to development and differentiation of colorectal cancer.