Objective To investigate the expression characteristics and clinical significance of P-selectin in benign and malignant hydatidiform mole tissues. Methods 50 cases of benign moles and 22 cases of malignant moles were selected. qRT-PCR technology is used to detect the expression level of P-selectin mRNA. Immunohistochemistry SP method and Western Blot technology comprehensively detected the expression level of P-Selectin protein. Results Compared with benign hydatidiform mole tissue, the relative expression of mRNA and protein and protein positive expression rate of P-selectin in malignant hydatidiform mole tissue were significantly higher (P<0.05). Conclusion P-selectin participates in the development of malignant hydatidiform mole at the gene and protein level, and is expected to become a diagnostic indicator for evaluating benign and malignant hydatidiform moles, providing new ideas for clinical targeted therapy.

Objective To check the differential expression of apoptosis-related factor Bid in adenocarcinoma of stomach tissue an and analyze its clinical significance. Methods 60 cases of adenocarcinoma of stomach tissues were the experimental group, and 55 cases of normal gastric mucosal epithelium tissue were the control group. The expression of Bid protein and mRNA was comprehensively detected by combined immunohistochemical SP method, Western blot and qRT-PCR technology. Results Compared with normal gastric mucosal epithelium tissue, the positive expression level of Bid protein and the relative expression level of mRNA in adenocarcinoma of stomach tissue are reduced, and the differences are statistically significant (P<0.05). In protein level and gene level, the Bid molecule expression level in adenocarcinoma of stomach tissues was related to the degree of differentiation, lymph node metastasis, clinical TNM stage and infiltration depth(P<0.05). Conclusion The apoptosis-related factor Bid plays a vital role in the occurrence and development of adenocarcinoma of stomach, and it may become a new target for the treatment of adenocarcinoma of stomach.

Objective:To investigate the expression and significance of integrin α5β1 and heparanase (HPA) in placental site trophoblastic tumor (PSTT) and choriocarcinoma tissue. Methods:Western blot was used to detect the integrin α5β1 and HPA protein expression in 20 cases choriocarcinoma tissue, 15 cases PSTT tissue and 40 cases normal villi. Results:The integrin α5β1 and HPA protein expression in choriocarcinoma tissue were obviously higher than PSTT tissue and normal villi (P<0.05); The integrin α5β1 and HPA protein expression in PSTT tissue were obviously higher than normal villi (P<0.05). Conclusions:Abnormal expression of integrin α5β1 and HPA maybe closely related to the development of choriocarcinoma and PSTT.