Objective To explore the relationship between the prognosis and vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), tissue plasminogen activator (tPA) in patients with endometriosis (EM). Methods Eighty-four EM patients, who were treated with conservative surgery, were selected as the study objects. According to the follow-up of 1 year after surgery, they were divided into recurrence group (11 cases) and non recurrence group (73 cases). The levels of VEGF, MMP-9 and tPA were measured before and after operation, and ROC curve was used to analyze the prognostic value of VEGF, MMP-9 and tPA in EM patients. Results Before surgery, there was no significant difference in the levels of VEGF, MMP-9, and tPA between the two groups of patients (P>0.05). On the 7th day after operation, the levels of VEGF and MMP-9 of the two groups were lower than that of preoperatively, while the levels of tPA were higher than that of preoperative, the differences were statistically significant (P<0.05). The levels of VEGF and MMP-9 were lower than that of patients in the recurrence group, and the tPA level was higher than that of patients in the recurrence group, the difference were statistically significant (P<0.05). ROC curve analysis showed that the AUC of serum VEGF, MMP-9, and tPA levels in evaluating the prognosis of EM patients were 0.783, 0.766, and 0.788, respectively, which had certain accuracy, and the evaluation value of the three-item combination (AUC=0.808, P<0.01) was higher. Conclusion The levels of VEGF, MMP-9, and tPA in patients with endometriosis are closely related to the prognosis, and the three combined tests are more valuable for their prognostic evaluation.

Objective: To investigate the effects of sericin on phosphatidylinositol-3-kinase (PI3K) mRNA expression in pancreas of type 2 diabetic rats. Methods: Male SD rats were randomly divided into three groups: normal control group, diabetic model group and sericin treatment group. The rat model of type 2 diabetes mellitus was established by feeding high-fat and high-sugar diet and intraperitoneal injection of streptozotocin (35mg/kg, twice). Then the rats in the sericin treatment group were given sericin (2.4g/kg/d) by gavage. Glucose oxidase method was used to detect the blood glucose of rats, and Real Time Q-PCR were used to detect the expression of PI3K mRNA in pancreas of rats in each group. Results: Compared with the normal control group, the blood glucose of diabetic rats increased significantly and the expression of PI3K mRNA in pancreas decreased significantly (P<0.05); Compared with diabetic rats, the blood glucose of sericin treated rats decreased significantly, and the expression of PI3K mRNA in pancreas increased significantly (P<0.05). Conclusions: Sericin may regulate insulin PI3K-Akt signaling pathway by increasing the expression of PI3K in pancreas, thus reducing blood glucose .

ObjectiveTo analyze the changes of insulin receptor substrate-1 (IRS-1) mRNA expression in pancreas of type 2 diabetic rats and the regulatory effects of sericin.MethodsMale SD rats were randomly divided into normal control group, diabetic model group and sericin medication group. The type 2 diabetic rats' model was established by high fat and high sugar feed combined streptozotocin injection. The rats in sericin medication group were lavaged with sericin (2.4g/kg) after the diabetic model was successfully established. Glucose oxidase method was used to detect the blood glucose of rats, Real Time Q-PCR to detect the IRS-1 mRNA expression in pancreas.ResultsCompared with rats in normal control group, the blood glucose of rats in diabetic model group increased obviously, the IRS-1 mRNA expression in pancreas decreased obviously (P<0.05). Compared with rats in diabetic model group, the blood glucose of rats in sericin medication group decreased significantly, the IRS-1 mRNA expression in pancreas increased significantly (P<0.05).ConclusionsSericin may reduce blood glucose by up regulating the expression of IRS-1 in pancreas.

Objective: To investigate the effects of sericin on expression of Bcl-2 protein in rats’ INS-1 cells. Methods: INS-1 cells were divided into normal control group, streptozotocin (STZ)-injuried group and sericin-treatment group. The INS-1 cells in normal control group were cultured with complete medium without any medicine; the INS-1 cells in STZ-injuried group were cultured with complete medium containing 10mmol/L STZ; the INS-1 cells in sericin-treatment group were cultured with complete medium containing 10mmol/L STZ and 300μg/ml sericin. Western Blotting was used to detect the expression of Bcl-2 protein in INS-1 cells of each group. Results: Compared with normal control group, the Bcl-2 protein expression in INS-1cells of STZ-injuried group decreased obviously (P<; 0.05). Compared with STZ-injuried group, the Bcl-2 protein expression in INS-1 cells of sericin-treatment group increased obviously (P<; 0.05). Conclusions: Sericin may inhibit beta cell apoptosis by up regulating the expression of Bcl-2 protein.