Objective To investigate the expression of HMGB1, RAGE, and IL-6 in patients with Henoch-Schonlein purpura (HSP), and to explore their roles in the development of HSP. Methods A total of 80 patients diagnosed with Henoch-Schonlein purpura at the Affiliated Hospital of Chengde Medical University from January 2022 to July 2024 were selected as the research objects. According to the results of the urine examination, patients were split into renal purpura group and non-renal purpura group, and a health examination population in the same period were selected as the control group. The expression of HMGB1, RAGE, and IL-6 was detected by ELISA. Non-parametric test and binary logistic regression model were utilized to analyze the data, and the ROC curve was adopted to analyze the predictive value of indicators for renal purpura. Results The expression levels of HMGB1 and IL-6 were highest in the renal purpura group, and the non-renal purpura group was higher than the control group (P<0.05). The expression levels of RAGE showed little difference between the renal purpura group and the non-renal purpura group (P>0.05), with both groups being higher than the control group (P<0.05). Binary logistic regression analysis shows that the increase in HMGB1 and IL-6 is statistically significant in the occurrence of renal purpura. The ROC curve indicates that the rise in HMGB1 and IL-6 demonstrates significant clinical significance for the early assessment of kidney damage. Conclusion HMGB1, RAGE, and IL-6 may be involved in the occurrence and development of HSP. HMGB1 is expected to be a potential target for the treatment of HSP. The combined detection of HMGB1 and IL-6 has high clinical value for early evaluation of the occurrence of renal purpura.

Objective The clinical significance of plasma CD89, PRDX1 and AOPP in the patients with non-renal henoch-schonlein purpura (HSP) and renal purpura was analyzed retrospectively, in order to provide references for the pathogenesis of HSP and early identification of renal purpura. Methods One hundred and four patients diagnosed with HSP in the Affiliated Hospital of Chengde Medical University from December 2020 to April 2023 were chosen as the study object. Patients were divided into non-renal purpura group (47 cases) and renal purpura group (57 cases) according to the results of urine test. Fifty-seven healthy people at the same time were chosen from the physical examination department as normal control group. The plasma levels of CD89, PRDX1 and AOPP in each group were measured by ELISA. Kruskal Wallis test and binary logistic regression were used for data comparison analysis and correlation analysis. ROC curve was applied to analyze the value of plasma CD89, PRDX1 and AOPP in the diagnosis of renal purpura. Results The plasma levels of CD89, PRDX1 and AOPP of renal purpura group were significantly higher than the levels of normal control group and non-renal purpura group, the results of non-renal purpura group were significantly higher than those of normal control group, there were statistically significant differences (P<0.05). Multivariate Logistic regression equation indicated that the effect of them on renal purpura was statistically significant. ROC curve shows that the combination of three factors has higher value in predicting the occurrence renal purpura. Conclusion Plasma CD89, PRDX1 and AOPP may be involved in the pathogenesis of HSP and renal purpura. The combination of plasma CD89, PRDX1 and AOPP has good clinical value in the diagnosis of renal purpura .

Objective To study the CD68 and VEGF expression in skin lesion tissue and their functions in pathogenesis of the erythema nodosum. Methods The expression of CD68 and VEGF in the skin lesions of 49 cases of erythema nodosum patients (disease group) and 10 cases of normal human (control group) were detected by MaxVisionTM immunohistochemical staining method. Results Compared with the normal control group(60.00%), the positive expression rate of CD68 in disease group(95.92%) was increased significantly, with statistical differences between two groups(P<0.01). Compared with the normal control group(60.00%), the positive expression rate of VEGF in disease group(95.92%) was increased significantly, with statistical differences between two groups (P<0.01). Conclusion Higher expression of CD68 and VEGF in skin lesions was concluded that active inflammatory cells and injury of endothelial cell were participated in pathogenesis of erythema nodosum.