Objective To investigate the effect of circular RNA hsa-circ-0001862 on the phenotype of tongue squamous cell carcinoma and its mechanism on the occurrence and development of tongue squamous cell carcinoma. Methods hsa-circ-0001862 plasmid was constructed, and the interaction relationship between hsa-circ-0001862 and miR-23a-3p was verified by double luciferase reporter gene and qRT-PCR. After targeted inhibition of hsa-circ-0001862, CCK8 assay and colony formation assay were used to detect the cell viability and colony formation ability of tongue squamous cell carcinoma cells. The migration and invasion ability of tongue squamous cell carcinoma cells were detected by scratch test and Transwell test. Apoptosis-related proteins were detected by Western blot to reflect the effect of hsa-circ-0001862 on the apoptosis of tongue squamous cell carcinoma cells. Results hsa-circ-0001862 and miR-23a-3p could interact, and their expression was negatively correlated in tongue squamous cell, carcinoma cells. In Tca-8113 cells, hsa-circ-0001862 inhibited cell proliferation, migration and invasion (P<0.01), and promoted cell apoptosis (P<0.01). Conclusion hsa-circ-0001862 interacts with miR-23a-3p, and hsa-circ-0001862 plays an inhibitory role in the development of tongue squamous cell carcinoma. hsa-circ-0001862 may be a new biomarker and target for the treatment of tongue squamous cell carcinoma.

Objective To explore the anti-inflammatory mechanism of loranthus parasiticus based on network pharmacology and molecular docking technology. Methods Active components and targets of loranthus parasiticus were gathered by TCMSP database and screened inflammatory targets by GeneCards, TTD and OMIM database. A “compound-target-disease” network was constructed with Cytoscape3.7.1. Protein interaction networks were mapped using String database and Clusterprofiler R Package was applied to enrich gene ontology GO and KEGG pathways of loranthus parasiticus intervention in inflammation. Molecular docking verification was carried out in AutoDockTools and AutoDockVina, and the results were visualized by PyMOL software. Results Forty-six active components and 418 potential targets of loranthus parasiticus, 696 targets of inflammation and 71 common targets of loranthus parasiticus and inflammation were collected. GO enrichment analysis and KEGG pathway enrichment analysis screened out 1703 items and 139 signaling pathways (P<0.05), mainly involving lipid and atherosclerosis, AGE-RAGE signaling pathway, TNF-α, IL-17 signaling pathway, Toll-like receptor signaling pathway, etc. The molecular docking results showed that quercetin had the strongest binding ability to AKT1. Conclusion Loranthus parasiticus may regulate multiple signaling pathways including IL-6, IL-1β, RelA, MAPK1 and AKT1 through quercetin, oleanolic acid and guajavarin to exert anti-inflammatory effects, providing reference for future studies.