Objective:To establish the method for simultaneous determination of rhamnosylvitexin, glucosylvitexin, hyperoside and vitexin content in Hawthorn leaf extract by quantitative analysis of multi-components by single marker. Methods:The relative correction factor of rhamnosylvitexin, hyperoside and vitexin were calculated by using glucosylvitexin as internal standard, and the content determination results were compared with the traditional external standard curve method. Results:The relative correction factor between glucosylvitexin and rhamnosylvitexin, hyperoside, vitexin were respectively 0.963, 1.116 and 1.034. There was no obvious difference about the rhamnosylvitexin, hyperoside and vitexin content between quantitative analysis of multi-components by single marker and traditional external standard curve method. Conclusions:The quantitative analysis of multi-components by single marker in this study can be used in simultaneous determination of 4 components in Hawthorn leaf extract.

Objective: To construct the HPLC fingerprint of Yixintong tablets. Methods: The HPLC was used to construct the fingerprint of 13 batches Yixintong tablets with the condition that the column was ZORBAX SB-C ₁₈ column (5µm, 4.6mm×250mm); the mobile phase was eluted with gradient by 0.1% formic acid-water, acetonitrile and tetrahydrofuran; the flow rate was 1.0ml/min; the detection wavelength was 350nm; the column temperature was 30℃; the sample volume was 10μl. The similarity evaluation software (2004A version) was used for similarity analysis. Results: The HPLC fingerprints of 13 batches Yixintong tablets calibrated 10 common peaks, and the similarity were all higher than 0.99. Conclusions: The method is simple, stable and feasible, and can be used to evaluate the quality of Yixintong tablets.

Objective: To comparatively analyze 4 different extraction parts (A.50% ethanol extract from hawthorn leaf,B.alcohol extract of hawthorn leaf after macroporous resin,C.ethyl acetate part of hawthorn leaf,D.n-butanol part of hawthorn leaf). of hawthorn leaves by establishing HPLC finger-print and detecting total flavonoids content. Methods: The fingerprint of hawthorn leaves was established by HPLC method using Diamonsil C ₁₈ (4.6 mm×250 mm, 5 μm) chromatographic column, acetonitrile-0.1% acetic acid-tetrahydrofuran as mobile phase with gradient elution, detection wavelength 320nm, flow rate of 0.9ml/min, and the column temperature was 30℃. The total flavonoids content was detected by ultraviolet spectrophotometry. Results: The common peaks of 4 extraction parts of hawthorn leaves in types and numbers had differences; The differences between extract C and the other three extracts was the largest. The total flavonoids content in extract B was the highest. Conclusions: This study can lay foundation for further development and utilization of hawthorn leaves extracts, and also can provide references for studying pharmacodynamic differences of different extraction parts of hawthorn leaves extracts.

Objective: To comparatively analyze the hawthorn leaves in south and north China by establishing HPLC fingerprints based on principal component analysis (PCA). Methods: HPLC was used to analyze 16 batches of hawthorn leaves from south (6 batches) and north (10 batches) China, and established HPLC fingerprints based on PCA. Results: 9 batches from north China and 6 batches from south China were selected to establish the UPLC fingerprints of hawthorn leaves based on PCA. The HPLC fingerprints of hawthorn leaves from north China had 16 common peaks, and the similarity was 0.808-0.972; the HPLC fingerprints of hawthorn leaves from south China had 23 common peaks, and the similarity was 0.923-0.979. Conclusions: The HPLC fingerprints of hawthorn leaves based on PCA in this study can objectively evaluate the differences of hawthorn leaves in south and north China, so it can provide references for clinical application of hawthorn leaves.

Objective: To establish hawthorn leaf fingerprint common patterns by using UPLC combined similarity calculation and clustering analysis, and provide reference for the quality evaluation of hawthorn leaves. Methods: The UPLC was used with the condition that the column was Waters CORTECS UPLC C ₁₈ column (3.0mm ×100mm,1.6μm); the mobile phase was eluted with gradient by acetonitrilee-0.1% formic acid; the flow rate was 0.2ml/min; the detection wavelength was 320nm; the column temperature was 30℃; the sample volume was 3μl. The UPLC fingerprint of 14 batches of hawthorn leaves was established by evaluation system of traditional Chinese medicine chromatographic fingerprint similarity (2004A version), and the similarity calculation and cluster analysis were carried out. Results: The 14 batches of hawthorn leaves were divided into Ⅰ, Ⅱ group by cluster analysis; and the hawthorn leaf fingerprint common pattern was established based on Ⅰ group. Conclusions: The method established in this study can provide reference for quality control of hawthorn leaves.