Objective To investigate the effects of resveratrol (RSV) on chronic kidney injury in high-fat mice by inhibiting endoplasmic reticulum (ER) stress response and its molecular mechanism. Methods Eighteen C57BL/6J mice were selected and randomly divided into three groups: the control group (CTRL group), high-fat group (HL group), and resveratrol group (RSV group), and a high-fat mouse model was established in HL and RSV group mice. RSV group was given a high-fat diet along with RSV 5 mg/(kg·d) lavage intervention. After 18 weeks, body weight, fasting blood glucose (FBG), low density lipoprotein cholesterol (LDL-C), low density lipoprotein cholesterol (HDL-C), uric acid (UA) and blood urea nitrogen (BUN) levels of mice in three groups were determined; the morphological changes of renal tissue in mice were observed with HE and PAS methods; the endoplasmic reticulum stress markers expression of CHOP, GRP78 protein and mRNA in kidney tissues were detected with western blot and RT-PCR methods. Results In RSV group, the body weight, FBG and LDL-C levels, serum UA and BUN levels were lower than those of HL group, while HDL-C level was higher than HL group, renal histopathological changes were improved, and the protein and mRNA expressions of ER stress markers CHOP and GRP78 were decreased. Conclusion RSV may improve chronic kidney injury in high fat diet mice by inhibiting the expression of endoplasmic reticulum markers CHOP and GRP78.

Objective To explore the mechanism of paeoniflorin in the treatment of neuropathic pain. Methods Forty male SD rats were randomly divided into the sham group, the chronic Constrictive injury(CCI) group, the MCC950 group and the albiflorin(AF) group, 10 rats in each group. The CCI model was established by CCI surgery. One day after operation, the corresponding drugs were given to each group and the model group was given the same amount of normal saline. The drugs were continuously given to each group until 11 days after operation.The paw withdrawal threshold (PWT) was measured by electronic Von Frey. The paw withdrawal latency(PWL) was measured by thermal pain stimulator. The caspase-1 activity was measured by caspase-1 activity assay kit. The IL-1β concentration was measured by ELISA kit. Results Compared with the CCI group, the PWT and PWL of AF group were significantly increased (P<0.001, P<0.01). NLRP3 level, Caspase-1 activity and IL-1β content were significantly decreased (P<0.01, P<0.001, P<0.05). Conclusion Albiflorin can effectively relieve neuropathic pain by controlling NLRP3 in spinal cord.