Objective: To investigate the expression and significance of Fas-associated death domain (FADD) protein and Caspase-7 protein in gastric adenocarcinoma Methods: The expression of FADD protein and Caspase-7 protein in 60 cases of gastric adenocarcinoma and 50 cases of normal gastric mucosa were detected by immunohistochemical SP method. The relationships between FADD protein, Caspase-7 protein and clinicopathological features of gastric adenocarcinoma, as well as the correlations between FADD protein and Caspase-7 protein in gastric adenocarcinoman were analyzed. Results: The positive products of FADD protein and Caspase-7 protein were all brownish yellow granules, mainly located in the cytoplasm. The positive expression rate of FADD protein and Caspase-7 protein in gastric adenocarcinoma were significantly lower than that in normal gastric mucosa tissues (38.3% vs 60.0%, 46.7% vs 68.0%, P<0.05). In gastric adenocarcinoma, the FADD protein and Caspase-7 protein expression were not correlated with age and gender (P>0.05); but related to the differentiation, TNM stage, lymph node metastasis and infiltration depth (P<0.05). Spearman correlation analysis showed that there had positive correlation between expression of FADD protein and caspase-7 protein in gastric adenocarcinoma (r=0.362, P<0.05). Conclusions: Low expression of FADD protein and Caspase-7 protein may play a synergistic role in the development of gastric adenocarcinoma.