Objective In order to investigate the application of anti-Dsg1and anti-Dsg3 antibody in diagnosis and treatment of patients, the level and changes of anti-Dsg1and anti-Dsg3 antibody in 18 patients with pemphigus and the relationship between the antibody index and the severity of the disease were analyzed. Methods 18 patients were diagnosed for pemphigus by direct immunofluorescence and pathology, and the patient's pathogenetic condition was assessed by ABSIS standard.The level of Dsg1 and Dsg3 of patients and nomals were detected by enzyme-linked immunosorbent assay(ELISA). Statistics was performed to analyze the relationship and significance of each index at last. Results Among the 18 patients, 13 were Dsg1 positive and 10 Dsg3 positive . The index of Dsg1 and Dsg3 after treatment were decreased than before, and the difference was statistically significant (P<0.05). Of the 20 nomals, 2 were Dsg1 positive and 1 was Dsg3 positive. The sensitivity of Dsg1 and Dsg3 antibody detection was 72.22% and 55.56% respectively, and the specificity was 90% and 95% respectively. The index of Dsg antibody was correlated with the ABSIS score (ALL P<0.05). Conclusion The Dsg antibody level of patients with pemphigus was correlated with the severity of the disease with high sensitivity and specificity. The detection of Dsg antibody level can be used for diagnosis and guidance of state and treatment of the disease.

Objective To study the features of clinical, dermoscopic and pathological of Seborrheic Keratosis and analyze their correlation. Methods Sixty-one patients with Seborrheic Keratosis diagnosed by histomathological examination and dermoscopy, who were admitted to the Affiliated Hospital of Chengde Medical University from March 2017 to December 2019, were selected to analyze the relationship between different clinical features, dermoscopy findings and pathological features of skin lesions. Results Under the dermoscope of fissures/ brain pattern appearance, comedo-like openings,milia-like cysts, the proportion of clinical papule, plaque and nodule was higher than that of macule, patch and verruca/papilla, and the differences were statistically significant (P<0.05). There were positive correlation between fissures/brain pattern appearance and hyperkeratosis and acanthosis (P<0.05). There were positive correlations between comedo-like openings, punctiform/hairpin blood vessels, milia-like cysts and acanthosis (P<0.05). Conclusion The diagnostic rate of seborrheic keratosis could be improved by the combination of dermoscopic examination and clinical features, and the accurate rate of diagnose of dermoscopy and pathology is highly consistent.